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Volume 18, Number 12—December 2012
CME ACTIVITY - Dispatch

Enterovirus 71–associated Hand, Foot, and Mouth Disease, Southern Vietnam, 2011

Truong Huu Khanh, Saraswathy Sabanathan, Tran Tan Thanh, Le Phan Kim Thoa, Tang Chi Thuong, Vu thi Ty Hang, Jeremy Farrar, Tran Tinh Hien, Nguyen van Vinh Chau, and H. Rogier van DoornComments to Author 
Author affiliations: Author affiliations: Children’s Hospital 1, Ho Chi Minh City, Vietnam (T.H. Khanh, L.P.K. Thoa, T.C. Thuong); Oxford University Clinical Research Unit, Oxford, UK (S. Sabanathan, T.T. Thanh, V.t.T. Hang, J. Farrar, T.T. Hien, H.R. van Doorn); Oxford University (S. Sabanathan, J. Farrar, T.T. Hien, H.R. van Doorn); Hospital for Tropical Diseases, Ho Chi Minh City (N.v.V. Chau)

Main Article

Table 2

Primers and probes used in study of EV71-associated hand, foot, and mouth disease, Children’s Hospital 1, Ho Chi Minh City, Vietnam, September 18–November 30, 2011*

Assay Primer and probe Sequence, 5′ → 3′ GenBank accession no. PCR product size, bp
EV71 real-time RT-PCR
 EV71-VP1-634F GGAGAACACAARCARGAGAAAGA AM490160.1
 Real-time analysis
EV71-VP1-743R ACTAAAGGGTACTTGGACTTVGA
EV71-VP1-TaqMan
 FAM-TGATGGGCACGTTCTCAGTGCG-BHQ1
CVA16 RT-PCR
 CoxA-VP1-526F AACCCATCTGTGTTTGTGAAAA JF317969.1
 110
CoxA-VP1-635R
 CCGAAGGTGGGATAACCAT
EV71 VP1 sequencing EV71-VP1-3F AGAYAGGGTGGCRGATGT AM490160.1 701
EV71-VP1-703R CTGAGAACGTGCCCATCA

*EV71, enterovirus 71; RT-PCR, reverse transcription PCR; VP, viral protein; FAM,  carboxyfluorescein; BHQ1, black hole quencher 1; CVA16, coxsackievirus A16; CoxA, coxsackievirus.

Main Article

Page created: November 19, 2012
Page updated: November 19, 2012
Page reviewed: November 19, 2012
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