Foodborne Transmission of Hepatitis E Virus from Raw Pork Liver Sausage, France
Christophe Renou , Anne-Marie Roque-Afonso, and Nicole Pavio
Author affiliations: Hôpital d’Hyères, Hyères, France (C. Renou); Hôpital Paul Brousse, Virologie, Villejuif, France (A.-M. Roque-Afonso); French Agency for Food, Environmental and Occupational Health and Safety, Maisons-Alfort, France (N. Pavio)
Figure. Phylogenetic analysis of partial open reading frame (ORF) 2 and ORF1 sequences of hepatitis E virus (HEV). Phylogenetic trees were constructed in MEGA6 software (http://www.megasoftware.net) by using the neighbor-joining method from a Kimura 2-parameter distance matrix based on partial nucleotide sequences of ORF2 (A) and ORF1 (B). Bootstrap values obtained from 500 resamplings are shown. Sequences were retrieved from the serum of a 45-year-old woman in France in whom hepatitis E was diagnosed in December 2013 and from frozen leftovers of the figatellu she had eaten in October 2013. Sequences obtained from the figatellu (GenBank accession nos. KJ603858 and KJ603860) were 100% nt identical to sequences obtained from the index case-patient (GenBank accession nos. KJ603859 and KJ603861). Reference sequences are indicated by their GenBank accession numbers. Scale bars indicate nucleotide substitutions per site.
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