Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 21, Number 3—March 2015
Dispatch

Rapid Detection of ESBL-Producing Enterobacteriaceae in Blood Cultures

Laurent Dortet, Laurent Poirel, and Patrice NordmannComments to Author 
Author affiliations: Institut National de la Santé et de la Recherche Médicale, Le Kremlin–Bicêtre, France (L. Dortet, L. Poirel, P. Nordmann); University of Fribourg, Fribourg, Switzerland, Department of Medicine (L. Poirel, P. Nordmann); Hôpital Fribourgeois-hôpital Cantonal, Fribourg (P. Nordmann)

Main Article

Table 3

MC values of several β-lactams and β-lactamase content for the extended-spectrum b-lactamase –producing Enterobacteriaceae in blood cultures*

Hospitalization unit a Species MICs (μg/mL)†
 β-Lactamase content c
CAZ CTX FEP CAZ/TZB CTX/TZB FEP/TZB SHV TEM CTX-M
Emergency Escherichia coli 2† 8 2 0.19 0.032 0.023 – – CTX-M-1
Rhumatology E. coli 1 12 2 0.19 0.032 0.016 – TEM-1 CTX-M-1
Emergency E. coli 32 48 8 0.75 0.25 0.064 SHV-12‡ – CTX-M-3
Emergency E. coli 1.5 16 6 0.38 0.094 0.064 – TEM-1 CTX-M-14
ICU E. coli 1 16 3 0.19 0.016 0.023 – TEM-1 CTX-M-14
Nephrology E. coli 0.5 12 1.5 0.094 0.047 0.016 – TEM-1 CTX-M-14
Nephrology E. coli 0.38 8 1.5 0.125 0.032 0.023 – TEM-1 CTX-M-14
Surgery E. coli 0.5 12 2 0.19 0.032 0.032 – TEM-1 CTX-M-14
ICU E. coli 8 96 6 0.19 0.032 0.032 – – CTX-M-15
ICU E. coli 1 16 0.75 0.094 0.032 0.047 – – CTX-M-15
Infectious diseases E. coli 12 128 12 0.25 0.094 0.064 – – CTX-M-15
Infectious diseases E. coli 16 128 12 0.19 0.094 0.094 – – CTX-M-15
Surgery E. coli 12 128 12 0.38 0.5 2 – – CTX-M-15
Urology E. coli 16 192 16 0.25 0.19 0.064 – TEM-1 CTX-M-15
Urology E. coli 12 192 8 0.25 0.125 0.064 – TEM-1 CTX-M-15
Infectious diseases E. coli 32 128 16 0.19 0.047 0.064 – TEM-1 CTX-M-15
Nephrology E. coli 48 6 1 0.38 0.19 0.094 SHV-12 TEM-1 –
Nephrology E. coli 48 6 1 0.38 0.19 0.094 SHV-12 TEM-1 –
Infectious diseases E. coli 12 128 8 0.19 0.047 0.023 – – CTX-M-15
Nephrology E. coli 16 128 12 0.25 0.094 0.047 – – CTX-M-15
ICU E. coli 256 256 192 32 24 2 – – CTX-M-15
ICU E. coli 8 96 6 0.19 0.064 0.064 – – CTX-M-15
ICU E. coli 12 128 6 0.125 0.064 0.125 – – CTX-M-15
Infectious diseases E. coli 32 256 24 0.5 0.19 0.094 – – CTX-M-15
Infectious diseases E. coli 32 256 24 0.5 0.19 0.094 – – CTX-M-15
Infectious diseases E. coli 256 256 16 0.5 0.016 0.094 – – CTX-M-15
Pneumology E. coli 32 256 16 0.5 0.094 0.032 – TEM-1 CTX-M-15
Emergency E. coli 256 12 1 0.094 0.032 0.016 – – CTX-M-27
ICU E. coli 1 12 1 0.094 0.023 0.023 – – CTX-M-27
Nephrology
 E. coli
 2
 2
 1.5
 0.094
 0.016
 0.016
–
 TEM-1
 CTX-M-55
ICU Klebsiella pneumoniae 3 32 4 0.125 0.023 0.032 – TEM-1 CTX-M-15
Infectious diseases K. pneumoniae 32 256 8 0.38 0.125 0.094 – TEM-1 CTX-M-15
ICU K. pneumoniae 128 256 64 1 0.25 0.19 LEN-5 – CTX-M-15
ICU K. pneumoniae 256 256 32 2 0.5 2 LEN-5 TEM-1 CTX-M-15
Nephrology K. pneumoniae 16 128 6 0.25 0.064 0.047 SHV-1 TEM-1 CTX-M-15
Hepatology K. pneumoniae 6 96 6 0.19 0.047 0.19 SHV-1 TEM-1 CTX-M-15
Nephrology K. pneumoniae 8 96 4 0.25 0.064 0.032 SHV-1 TEM-1 CTX-M-15
ICU K. pneumoniae 48 256 24 0.5 0.064 0.064 SHV-1 TEM-1 CTX-M-15
Hepatology K. pneumoniae 16 64 6 0.5 0.25 0.125 SHV-11 TEM-1 CTX-M-15
Hepatology K. pneumoniae 12 48 6 0.5 0.19 0.094 SHV-11 TEM-1 CTX-M-15
ICU K. pneumoniae 12 128 12 0.38 0.047 0.094 SHV-11 TEM-1 CTX-M-15
Nephrology K. pneumoniae 48 256 16 0.5 0.19 0.5 SHV-11 TEM-1 CTX-M-15
ICU
 K. pneumoniae
 256
 256
 24
 0.25
 0.047
 0.064
SHV-28
 TEM-1
 CTX-M-15
ICU Enterobacter cloacae 96 256 32 1 1 0.25 – – CTX-M-15
Nephrology E. cloacae 12 192 12 0.25 0.125 0.047 – TEM-1 CTX-M-15
ICU
 E. cloacae
 12
 128
 6
 0.25
 0.047
 0.047
SHV-1
 TEM-1
 CTX-M-15
Emergency Citrobacter freundii 16 12 2 2 1 0.5 – TEM-3 +TEM-1 CTX-M-55

ICU, intensive care unit, CAZ, ceftazidime; CTX, cefotaxime; FEP, cefepime; TZB, tazobactam; –, negative PCR result.
†Numbers in bold text indicate MIC values reported in the range of susceptibility according to Clinical Laboratory Standards Institute guidelines as updated in 2014 (susceptible means MIC ≤1 μg/mL, ≤4 μg/mL and ≤2 μg/mL for cefotaxime, ceftazidime and cefepime, respectively).
‡Bold, underlined text indicates ESBL names.

Main Article

Page created: February 18, 2015
Page updated: February 18, 2015
Page reviewed: February 18, 2015
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external