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Volume 21, Number 3—March 2015

Lack of Effect of Lamivudine on Ebola Virus Replication

Lisa Hensley, Julie Dyall, Gene G. Olinger, and Peter B. JahrlingComments to Author 
Author affiliations: National Institutes of Health, Frederick, Maryland, USA

Main Article


Inhibitory effects of test compounds on Ebola virus replication*

Virus variant or subtype Cell type Lamivudine EC50, μmol/L† Toremifene EC50 ± SD, μmol/L†
EBOV/Kik Vero E6 >80 5.7 ± 0.7
EBOV/Kik Vero E6 >320 12.0 ± 1.0
EBOV/Gue Vero E6 >320 ≈8
EBOV/Kik HepG2 >80 1.6 ± 0.1
EBOV/Kik HepG2 >320 5.5 ± 0.1
EBOV/Kik Macrophages >320 ≈25
EBOV/Kik Macrophages >320 18.3 ± 0.8
HIV-1 Macrophages 0.002‡ ND
HIV-1 Monocytes 0.69‡ ND
HIV-1 (multiple subtypes) PBMC 0.002–2.5‡ ND
HBV HepG2 (2.2.15) 0.002§ ND

*EBOV, Ebola virus; EC50, 50% effective concentration; BMC, peripheral blood mononuclear cells; ND, not done; HBV, hepatitis B virus.
†EC50s were determined by using an EBOV ELISA with antibodies against glycoprotein or viral matrix protein as described (3).
‡Data from Schinazi (4).
§Data from Kruining et al. (5).

Main Article

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Page created: February 18, 2015
Page updated: February 18, 2015
Page reviewed: February 18, 2015
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