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Volume 21, Number 7—July 2015
Dispatch

Severe Pediatric Adenovirus 7 Disease in Singapore Linked to Recent Outbreaks across Asia

Oon Tek NgComments to Author , Koh Cheng Thoon, Hui Ying Chua, Natalie Woon Hui Tan, Chia Yin Chong, Nancy Wen Sim Tee, Raymond Tzer Pin Lin, Lin Cui, Indumathi Venkatachalam, Paul Anantharajah Tambyah, Jonathan Chew, Raymond Kok Choon Fong, Helen May Lin Oh, Prabha Unny Krishnan, Vernon Jian Ming Lee, Boon Huan Tan, Sock Hoon Ng, Pei Jun Ting, Sebastian Maurer-Stroh, Vithiagaran Gunalan, and Wei Xin Khong

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Figure 2

Phylogenetic analysis of adenovirus type 7 (Ad7) sequences from this study based on sequenced Ad7 hexon gene hypervariable regions 1–6 (Ad7 reference Gomen AY594255 hexon gene nt 324 to 1123). The phylogenetic relationships between Ad7 isolates in this study were inferred by using the maximum-likelihood method based on the Tamura-Nei model (8). Initial trees for the heuristic search were obtained by applying the neighbor-joining method to a matrix of pairwise distances estimated by using the max

Figure 2. Phylogenetic analysis of adenovirus type 7 (Ad7) sequences from this study based on sequenced Ad7 hexon gene hypervariable regions 1–6 (Ad7 reference Gomen AY594255 hexon gene nt 324 to 1123). The phylogenetic relationships between Ad7 isolates in this study were inferred by using the maximum-likelihood method based on the Tamura-Nei model (8). Initial trees for the heuristic search were obtained by applying the neighbor-joining method to a matrix of pairwise distances estimated by using the maximum-composite likelihood approach. Tree is drawn to scale; branch lengths are measured in the number of substitutions per site, the rate of which was assumed to be uniformly distributed. The analysis involved 63 nt sequences. Green indicates isolates from military personnel, dark blue indicates isolates from patients in National University Hospital, pink indicates isolates from patients in KK Women’s and Children’s Hospital, and light blue indicates isolates from patients in Changi General Hospital. The sequence labeled as “AY594255_7_Gomen (USA 1954)” represents 1 of the published Gomen sequences (AY594255). “_7” is added to GenBank accession numbers to denote that these are Ad7 sequences. All other strains shown are published Ad7 reference isolates. Phylogenetic analyses were conducted by using MEGA6 (9). KK341 and KK342 had a single thymine-to-cytosine mutation (Ad7 reference Gomen AY594255 hexon gene nt 780). The remaining 41 isolates from Singapore had 100% nucleotide identity.

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