Volume 21, Number 8—August 2015
Seasonal Patterns of Buruli Ulcer Incidence, Central Africa, 2002–2012
To determine when risk for Buruli ulcer is highest, we examined seasonal patterns in a highly disease-endemic area of Cameroon during 2002–2012. Cases peaked in March, suggesting that risk is highest during the high rainy season. During and after this season, populations should increase protective behaviors, and case detection efforts should be intensified.
Buruli ulcer (BU) is a severe infection caused by Mycobacterium ulcerans. Most affected are rural populations living in tropical areas with abundant wetlands (1). BU causes extensive, damaging skin lesions and often results in severe disabilities. Of the 4,000 cases reported to the World Health Organization by 14 countries in 2011, >95% originated in African countries around the Gulf of Guinea (2).
Much remains unknown about the mode of M. ulcerans transmission and the epidemiology of BU (1). Specifically, although the spatial distribution of BU in several settings has been addressed (3,4), most studies have examined only temporal variations of BU incidence in terms of yearly trends (5). Several observational studies have reported seasonal changes in the monthly number of cases and have hypothesized that cases are linked with rainfall variation (6–8). One spatiotemporal study in Australia showed that BU incidence was associated with rainfall variability with a 5-month lag and with total rainfall with a 19-month lag (4). However, none of these studies provided quantitative evidence of seasonal changes in BU incidence and their relationship with seasonal environmental changes. Indeed, a formal demonstration of such evidence requires a sufficiently long time series, large numbers of cases from a defined source population, and use of signal analysis techniques adapted to the constraints of BU disease surveillance and environmental data (9) (Technical Appendix). Therefore, we investigated the seasonality of BU case incidence during 2002–2012 in Akonolinga District, located in the highly BU-endemic region of the Nyong River valley in Centre Region, Cameroon.
Relying on previous spatial analysis of BU incidence in Akonolinga District, we analyzed a series of cases that occurred in the highest BU-risk area of the district, located along the Nyong River upstream of Akonolinga (3). This area includes 24,469 inhabitants of the town of Akonolinga and 24 surrounding villages. We analyzed 562 new cases of BU that originated in this area from January 2002 through May 2012, after aggregation by month of diagnosis. Biological confirmation was obtained from the National Reference Centre for Mycobacteria for 354 (63%) cases. The BU incidence rate remained stable over the 10-year period at 2.2 cases/1,000 person-years.
Median BU incidence peaked in March, and a second peak occurred in September (Technical Appendix Figure 2), but monthly medians did not differ significantly (Kruskal-Wallis test, p = 0.149). Given the specificities (nonstationarity) of the BU case series, wavelet analysis was the appropriate method for analysis (Technical Appendix). A 1-year periodic signal was identified in the BU-case time series from 2005 to 2011, and this periodicity was statistically significant from mid-2005 to the beginning of 2009 (Figure 1).
Next, we analyzed the links between BU and seasonal changes by using wavelet association and phase analyses between BU case incidence and total monthly rainfall (in mm) or mean Nyong River flow (in cubic meters per second). Strong seasonality was found in the series of monthly total rainfall and of monthly mean Nyong River flow; a 1-year period and a weaker 6-month period corresponded to the 2 rainy seasons separated by a period of lesser rainfall (the small dry season, mid-July to mid-August) (Figure 1; Technical Appendix Figure 3). Because of its shape, the wavelet detected yearly rainfall oscillations between a minimum in December (dry season) and a maximum in July (the middle of the rainy period) instead of the maximal rainfall months of October and November (Technical Appendix Figure 3).
We assessed the association of the incident case signal with environmental variables (Technical Appendix). The 1-year periodic signal of the BU case series was associated with Nyong River flow from the end of 2005 to the end of 2009 (Figure 1) and with rainfall from the end of 2005 to the beginning of 2011 (Technical Appendix Figure 3). Under the assumption that changes in the environment preceded changes in BU incidence, phase analysis indicated that cases lagged 6 months behind Nyong River flow oscillations (Figure 1). When the 2 signals were associated, a 9-month lag behind rainfall oscillations was observed (Technical Appendix Figure 3).
In the BU-endemic focus of Akonolinga, Cameroon, significant 1-year seasonal variations in BU incidence occur. The incubation period for BU has been estimated to be ≈4.5 months when data from Australia are used (10) and ≈3 months when data from Uganda are used (7). The median delay between symptom onset and health care seeking was reported to be 5 weeks in Akonolinga (interquartile range 3–12 weeks), yielding a delay between infection and diagnosis of 5–6 months. Given this delay and a finding of BU diagnosis peaks during March–April, the number of infections would therefore be highest from August through October (Figure 2). Such a pattern was observed in the 1970s in Uganda (6,7) and Cameroon (11) and more recently in Côte d’Ivoire (5). In low BU-endemicity French Guiana, an overseas territory located near Brazil at the same latitude as Cameroon, periodic peaks after the 2 rainy seasons have been reported (12).
Variations in BU incidence result from variations in population exposure (13) combined with variations in environmental presence of M. ulcerans (14). We hypothesize that one of the main drivers of these variations is the seasonal flooding of the Nyong River, which rises 3–5 m from April through November, creating temporary bodies of water and swamps on a vast surface, deeply affecting the ecosystem.
Although M. ulcerans was identified year-round in specific environments such as permanent swamps, its presence and abundance were maximal during the rainy months, July–October (14) (Figure 2). Prevalence of M. ulcerans in rivers was high at the beginning of the rainy season and was high in flooded areas during the following small dry season and high rainy season (14).
Human activity patterns follow these seasonal changes, resulting in seasonal variations in exposure to M. ulcerans. In Uganda, the contribution of permanent swamps to BU risk and the increased risk associated with temporary swamps during the rainy season have been documented (8). According to residence, age, and/or sex, the inhabitants of the Akonolinga district face varying exposures to aquatic environments; during the period identified as high risk, populations frequent seasonally flooded environments for water collection, fishing, and harvest of dry season cultures (Figure 2) (15).
During the study period, the intensity of the association between BU incidence and rainfall or Nyong River flow varied. The seasonal signal was detected over 5 consecutive years and was strongest when yearly variations in the Nyong River flow were lower (2005, 2006, 2008), which could indicate transient forcing of BU incidence by seasonal phenomena. Assessment of the effects of lower frequency climatic events, such as El Niño Southern Oscillation, is needed. In French Guiana, where BU endemicity is low, such events were shown to affect BU incidence dynamics (12).
We showed that BU incidence in this region varies significantly by season and linked these variations to the fluctuations of M. ulcerans occurrence in the environment, which are probably driven by the dynamics of freshwater ecosystems of the Nyong River. In Akonolinga, during the high rainy season when risk for M. ulcerans transmission seems to be highest, populations should increase their protective behaviors, and case detection efforts should be intensified in subsequent months to ensure early diagnosis and access to care.
Dr. Landier specializes in infectious diseases epidemiology. While working on his PhD degree at the Institut Pasteur in Paris, he focused on BU epidemiology and M. ulcerans transmission in Central Africa.
We thank the staff of the Akonolinga district hospital, who provided diagnoses and delivered treatment to BU patients, and the staff of the Service de Mycobactériologie at Centre Pasteur du Cameroun, who provided laboratory confirmation of diagnoses.
- Merritt RW, Walker ED, Small PLC, Wallace JR, Johnson PDR, Benbow ME, Ecology and transmission of Buruli ulcer disease: a systematic review. PLoS Negl Trop Dis. 2010;4:e911. DOIPubMedGoogle Scholar
- World Health Organization. Summary data. WHO meeting on Buruli ulcer control and research, 2013 Mar 25–27; Geneva; 2012 [cited 2013 Dec 12]. http://www.who.int/buruli/Summary_data_and_new_target_2013.pdf
- Landier J, Gaudart J, Carolan K, Lo Seen D, Guégan J-F, Eyangoh S, Spatio-temporal dynamics and landscape-associated risk of Buruli ulcer in Akonolinga, Cameroon. PLoS Negl Trop Dis. 2014;8:e3123. DOIPubMedGoogle Scholar
- van Ravensway J, Benbow ME, Tsonis AA, Pierce SJ, Campbell LP, Fyfe JAM, Climate and landscape factors associated with Buruli ulcer incidence in Victoria, Australia. PLoS ONE. 2012;7:e51074. DOIPubMedGoogle Scholar
- Marston BJ, Diallo MO, Horsburgh CR, Diomande I, Saki MZ, Kanga JM, Emergence of Buruli ulcer disease in the Daloa region of Côte d’Ivoire. Am J Trop Med Hyg. 1995;52:219–24 .PubMedGoogle Scholar
- Revill WD, Barker DJP. Seasonal distribution of mycobacterial skin ulcers. Br J Prev Soc Med. 1972;26:23–7 .PubMedGoogle Scholar
- Uganda Buruli Group. Epidemiology of Mycobacterium ulcerans infection (Buruli ulcer) at Kinyara, Uganda. Trans R Soc Trop Med Hyg. 1971;65:763–75 . DOIPubMedGoogle Scholar
- Barker DJP, Carswell JW. Mycobacterium ulcerans infection among tse-tse control workers in Uganda. Int J Epidemiol. 1973;2:161–5. DOIPubMedGoogle Scholar
- Cazelles B, Chavez M, De Magny GC, Guégan J-F, Hales S. Time-dependent spectral analysis of epidemiological time-series with wavelets. J R Soc Interface. 2007;4:625–36.PubMedGoogle Scholar
- Trubiano JA, Lavender CJ, Fyfe JA, Bittmann S, Johnson PD. The incubation period of Buruli ulcer (Mycobacterium ulcerans infection). PLoS Negl Trop Dis. 2013;7:e2463. DOIPubMedGoogle Scholar
- Ravisse P. Skin ulcer caused by Mycobacterium ulcerans in Cameroon. I. Clinical, epidemiological and histological study [in French]. Bull Soc Pathol Exot. 1977;70:109–24.PubMedGoogle Scholar
- Morris A, Gozlan RE, Hassani H, Andreou D, Couppié P, Guégan JF. Complex temporal climate signals drive the emergence of human waterborne disease. Emerging Microbes & Infections. 2014;3:e56.
- Pouillot R, Matias G, Wondje CM, Portaels F, Valin N, Ngos F, Risk factors for Buruli ulcer: a case control study in Cameroon. PLoS Negl Trop Dis. 2007;1:e101. DOIPubMedGoogle Scholar
- Garchitorena A, Roche B, Kamgang R, Ossomba J, Babonneau J, Landier J, Mycobacterium ulcerans ecological dynamics and its association with freshwater ecosystems and aquatic communities: results from a 12-month environmental survey in Cameroon. PLoS Negl Trop Dis. 2014;8:e2879. PLoS Negl Trop Dis. 2014;8:e2879 .DOIPubMedGoogle Scholar
- Giles-Vernick T, Owona-Ntsama J, Landier J, Eyangoh S. The puzzle of Buruli ulcer transmission, ethno-ecological history and the end of “love” in the Akonolinga district, Cameroon. Soc Sci Med. 2015;129:20–7. DOIPubMedGoogle Scholar
FiguresCite This Article
Table of Contents – Volume 21, Number 8—August 2015
|EID Search Options|
|Advanced Article Search – Search articles by author and/or keyword.|
|Articles by Country Search – Search articles by the topic country.|
|Article Type Search – Search articles by article type and issue.|
Please use the form below to submit correspondence to the authors or contact them at the following address:
Jordi Landier, Unité d'Epidémiologie des Maladies Emergentes, Institut Pasteur, 25–28 Rue du Dr Roux, 75015 Paris, France