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Volume 22, Number 2—February 2016
Dispatch

Frequency and Distribution of Rickettsiae, Borreliae, and Ehrlichiae Detected in Human-Parasitizing Ticks, Texas, USA

Elizabeth A. Mitchell, Phillip Williamson, Peggy M. Billingsley, Janel P. Seals, Erin E. Ferguson, and Michael S. AllenComments to Author 
Author affiliations: University of North Texas Health Science Center, Fort Worth, Texas, USA (E.A. Mitchell, P.C. Williamson, P.M. Billingsley, J.P. Seals, E.E. Ferguson, M.S. Allen); Creative Testing Solutions, Tempe, Arizona, USA (P.C. Williamson); University of Utah, Salt Lake City, Utah, USA (P.M. Billingsley)

Main Article

Table 1

Primers used for screening of human-parasitizing tick specimens, Texas, USA, October 1, 2008–September 30, 2014

Primer name Gene Primer sequence, 5′ → 3′ Specificity Amplicon, bp Reference
Borrelia spp.
FlaLL flaB ACATATTCAGATGCAGACAGAGGT Genus 664 (3)
FlaRL flaB GCAATCATAGCCATTGCAGATTGT Genus (3)
FlaLS flaB AACAGCTGAAGAGCTTGGAATG Genus 330 (3)
FlaRS flaB CTTTGATCACTTATCATTCTAATAGC Genus (3)
BL-Fla 522F flaB GGTACATATTCAGATGCAGACAGAGGG B. lonestari 660 (2)
BL-Fla 1182R flaB GCACTTGATTTGCTTGTGCAATCATAGCC B. lonestari (2)
BL-Fla 662F flaB CTGAAGAGCTTGGAATGCAACCTGC B. lonestari 198 (2)
BL-Fla 860R flaB GAGCTAATCCCACCTTGAGCTGG B. lonestari (2)
BL-16S 227F 16S TCACACTGGAACTGAGATACGGTCC Genus 693
 (2)
BL-16S 920R
 16S
 GAATTAAACCACATGCTCCACCGC
 Genus
 (2)
Rickettsia spp.
Rr.190 70P rompA ATGGCGAATATTTCTCCAAAA Genus 532 (4)
Rr.190 602N rompA AGTGCAGCATTCGCTCCCCCT Genus (4)
BG1–21 rompB GGCAATTAATATCGCTGACGG Genus 650
 (5)
BG2–20
 rompB
 GCATCTGCACTAGCACTTTC
 Genus
 (5)
Ehrlichia spp.
Ehr DSB 330F dsb GATGATGTCTGAAGATATGAAACAAAT Genus 398 (6)
Ehr DSB 728R dsb CTGCTCGTCTATTTTACTTCTTAAAGT Genus (6)
Ehr map1F map1 ATTTTTACCTGGTGTGTCCTTTTCTGA Genus 873
 (7)
Ehr map1R map1 CCTTCCTCCAATTTCTATACC Genus (7)
Ehr Pmap2F map1 GACACCAAGGCAGTATACGG Genus (7)
Ehr Pmap2R
 map1
 CTAAGTCAGTACCAATACCTGCAC
 Genus
 (7)
Tick DNA
16S-1 mt16S CCGGTCTGAACTCAGATCAAG Unknown 300 (8)
16S+2 mt16S TTGGGCAAGAAGACCCTATGAA Unknown (8)

Main Article

Page created: January 15, 2016
Page updated: January 15, 2016
Page reviewed: January 15, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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