Emanuela Giombini, Martina Rueca, Walter Barberi, Anna Paola Iori, Concetta Castilletti, Paola Scognamiglio, Francesco Vairo, Giuseppe Ippolito, Maria Rosaria Capobianchi, and Maria Beatrice Valli
Figure 2. Phylogenetic tree of partial viral protein 1 sequences of 279 nt (nt positions 2581–2859, reference sequence GenBank accession no.AY426531.1), sequences from cerebrospinal fluid (accession no. MF061604) and oropharyngeal swab sample (accession no. MF061605) from a woman who died of fatal neurologic disease associated with enterovirus-D68 infection (indicated in red) in the context of 918 enterovirus-D68 global sequences retrieved from the National Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/). The main figure shows the whole maximum-likelihood tree (1,000 bootstrap replicas), generated by using the HKY+GI (Hasegawa-Kishino-Yano + gamma distribution invariant sites) model; the inset shows an enlargement of subclade B3 containing the sequences from the patient reported here and patients involved in the 2016 epidemic in the Netherlands: the closest sequence is KX685068.1_Netherlands_2016 (98% identity, distance: 180 substitutions/104 positions). Red dots indicate nodes with bootstrap value >70.