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Volume 23, Number 12—December 2017
Dispatch

Phylogenetic Characterization of Crimean-Congo Hemorrhagic Fever Virus, Spain

Eva Ramírez de Arellano, Lourdes Hernández, M. José Goyanes, Marta Arsuaga, Ana Fernández Cruz, Anabel Negredo1, and María Paz Sánchez-Seco1Comments to Author 
Author affiliations: National Center of Microbiology Arbovirus and Imported Viral Diseases Laboratory, Madrid, Spain (E. Ramírez de Arellano, L. Hernández, A. Negredo, M. Paz Sánchez-Seco); Gregorio Marañón Hospital, Madrid (M.J. Goyanes, A. Fernández Cruz); La Paz Hospital, IdiPaz, Madrid (M. Arsuaga)

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Figure

Phylogenetic analysis of Crimean-Congo hemorrhagic fever virus from patient in Spain, 2016, compared with reference sequences. A) Small segment (1,450 bp); B) medium segment (4,497 bp); C) large segment (11,829 bp). Trees were generated with the neighbor-joining method with Kimura 2-parameter distances by using MEGA version 5.1 (http://www.megasoftware.net). Bootstrap confidence limits were calculated on the basis of 1,000 replicates; numbers on branches indicate bootstrap results. Triangles ind

Figure. Phylogenetic analysis of Crimean-Congo hemorrhagic fever virus from patient in Spain, 2016, compared with reference sequences. A) Small segment (1,450 bp); B) medium segment (4,497 bp); C) large segment (11,829 bp). Trees were generated with the neighbor-joining method with Kimura 2-parameter distances by using MEGA version 5.1 (http://www.megasoftware.net). Bootstrap confidence limits were calculated on the basis of 1,000 replicates; numbers on branches indicate bootstrap results. Triangles indicate newly sequenced strain from Spain described in this article; other sequences are named by GenBank accession number, strain, geographic origin, and sampling year. Genotypes at right are named according to Carrol et al. (4); brackets indicate equivalent group nomenclature according to Chamberlain et al. (1). Roman numerals indicate geographic locations: I, West Africa (Africa 1); II, Central Africa (Africa 2); III, South and West Africa (Africa 3); IV, Middle East/Asia, divided into 2 groups, Asia 1 and Asia 2 (1); V, Europe/Turkey (Europe 1); VI, Greece (Europe 2). Scale bars indicate nucleotide substitutions per site.

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1These authors contributed equally to this article.

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