Volume 25, Number 6—June 2019
Research
Mass Die-Off of Saiga Antelopes, Kazakhstan, 2015
Sasan Fereidouni, Graham L. Freimanis, Mukhit Orynbayev, Paolo Ribeca, John Flannery, Donald P. King, Steffen Zuther, Martin Beer, Dirk Höper, Aidyn Kydyrmanov, Kobey Karamendin, and Richard Kock
Table 5
Summary of the most relevant results obtained by RIEMS analyses of the datasets (sequenced from shotgun libraries generated from random primed cDNA) used in an investigation of a mass die-off of saiga antelopes, Kazakhstan, 2015
| Organism | No. (%) reads |
|||
|---|---|---|---|---|
| Sample 2* (lib01416) | Sample 5* (lib01417) | Sample 7 (lib01418) | Sample 8 (lib01419) | |
| Input reads | 411,640 (100) | 376,210 (100) | 372,387 (100) | 354,958 (100) |
| Quality filtered reads† | 12,786 (3.1) | 10,793 (2.9) | 11,559 (3.1) | 10,895 (3.1) |
| Unclassified reads† | 1,776 (0.43) | 1,520 (0.40) | 1,626 (0.44) | 1,494 (0.42) |
| Classified reads† | 397,078 (96.5) | 363,897 (96.7) | 359,202 (96.5) | 342,569 (96.5) |
| Host‡ | 64,618 (16.3) | 4,770 (1.3) | 3,414 (1.0) | 4,784 (1.4) |
| Pasteurellaceae‡ | 317,009 (79.8) | 345,893 (95.1) | 339,484 (94.5) | 324,770 (94.8) |
*Samples 2 and 5 were also tested by using high-throughput sequencing at the Pirbright Institute.
†Percentage is of the number of input reads.
‡Percentage is of the number of classified reads.
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