Volume 25, Number 6—June 2019
Research Letter
Comparative Analysis of Whole-Genome Sequence of African Swine Fever Virus Belgium 2018/1
Jan H. Forth, Marylène Tignon, Ann Brigitte Cay, Leonie F. Forth, Dirk Höper, Sandra Blome, and Martin Beer
Table
Differences in the whole-genome sequence of ASFV-Belgium2018/1 and the improved ASFV-Georgia2007/1 reference genome (indicated in bold and underlined)*
| Position | Type | Difference | ASFV Georgia 2007/1† | ASFV Belgium 2018/1† | Coverage | Gene | Synonymous/ nonsynonymous | ORF change |
|---|---|---|---|---|---|---|---|---|
| 121 | Transversion | G121C | AAGAT | AACAT | 44 | ITR | NA | NA |
| 129 | Transversion | A129T | AAATA | AATTA | 47 | ITR | NA | NA |
| 479 | Variation | +T | 9 × T | 10 × T | 767 | ITR | NA | NA |
| 1393 | Variation | +C | 9 × C | 10 × C | 1375 | NA | NA | NA |
| 6786 | Variation | –T | 9 × T | 8 × T | 1755 | NA | NA | NA |
| 7061 | Transition | C7061T | CCCAG | CCTAG | 1556 | MGF 110–1L | Trp197Stop | Shortened by 18 codons |
| 15688 | Variation | +3C | 17 × C | 20 × C | 127 | MGF 110–13Lb | Frameshift | Enlarged by 1 codon |
| 17640 | Variation | +2G | 10 × G | 12 × G | 1117 | NA | NA | NA |
| 17854 | Variation | +G | 9 × G | 10 × G | 1304 | NA | NA | NA |
| 19807 | Variation | –G | 7 × G | 6 × G | 1238 | NA | NA | NA |
| 20017 | Variation | –2G | 16 x G | 14 × G | 832 | ASFV G ACD 00350 | Frameshift | Truncation |
| 21815 | Variation | +2G | 9 × G | 11 × G | 1184 | NA | NA | NA |
| 27439 | Variation | –T | 10 × T | 9 × T | 1895 | NA | NA | NA |
| 44586 | Transition | A44586G | TGAAA | TGGAA | 1292 | MGF 505–9R | Lys323Glu | NA |
| 73282 | Variation | –T | 9 × T | 8 × T | 1241 | NA | NA | NA |
| 134524 | Transition | T134524C | CATTT | CACTT | 1665 | NP419L | Asp414Ser | NA |
| 145080 | Transition | G145080A | GAGGC | GAAGC | 1266 | D117L | Pro84Leu | NA |
| 170827 | Transversion | T170827A | GATGG | GAAGG | 1263 | I267L | Ile195Phe | NA |
| 190138 | Variation | +A | 9 × A | 10 × A | 3021 | DP60R | Frameshift | 22 changed codons |
| 173402 | Repeat integration | +TATATAGGAA | NA | +TATATAGGAA | 1731 | NA | NA | NA |
| 190478 | Transversion | T190478A | TATTT | TAATT | 49 | ITR | NA | NA |
| 190486 | Transversion | C190486G | ATCTT | ATGTT | 49 | ITR | NA | NA |
*Differences are show in bold and underlined. ASFV, African swine fever virus; ITR, inverted terminal repeat; NA, not applicable; ORF, open reading frame.
†International Nucleotide Sequence Database Collaboration accession nos: ASFV Georgia 2007/1, FR682468.2; ASFV Belgium 2018/1, LR536725.
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Page updated: May 20, 2019
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