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Volume 26, Number 1—January 2020
Research

Preclinical Detection of Prions in Blood of Nonhuman Primates Infected with Variant Creutzfeldt-Jakob Disease

Luis Concha-Marambio1, Marcelo A. Chacon, and Claudio SotoComments to Author 
Author affiliations: University of Texas, Houston, Texas, USA (L. Concha-Marambio, M.A. Chacon, C. Soto); Universidad de los Andes, Santiago, Chile (L. Concha-Marambio, C. Soto)

Main Article

Figure 1

Amplification of macaque-adapted vCJD prions by PMCA. BH from 3 macaques peripherally infected with macaque-adapted vCJD was serially diluted and amplified by 3 rounds of PMCA, using BH from transgenic mice expressing human normally expressed prion protein with methionine at codon 129 (TgHu129M) as substrate. Human BH from a vCJD patient was analyzed as positive control. After completion of the 3 rounds of PMCA, samples were digested with 50 μg/mL of proteinase K and analyzed by Novex SDS-PAGE (

Figure 1. Amplification of macaque-adapted vCJD prions by PMCA. BH from 3 macaques peripherally infected with macaque-adapted vCJD was serially diluted and amplified by 3 rounds of PMCA, using BH from transgenic mice expressing human normally expressed prion protein with methionine at codon 129 (TgHu129M) as substrate. Human BH from a vCJD patient was analyzed as positive control. After completion of the 3 rounds of PMCA, samples were digested with 50 μg/mL of proteinase K and analyzed by Novex SDS-PAGE (https://www.thermofisher.com). N refers to transgenic mouse normal BH without proteinase K treatment, which was used as a migration control. BH, brain homogenate; PMCA, protein misfolding cyclic amplification; vCJD, variant Creutzfeldt-Jakob disease.

Main Article

1Current affiliation: Amprion, Inc., San Diego, California, USA.

Page created: December 17, 2019
Page updated: December 17, 2019
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