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Volume 26, Number 4—April 2020
Research Letter

Geographic Distribution of Raccoon Roundworm, Baylisascaris procyonis, Germany and Luxembourg

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Mike HeddergottComments to Author , Peter Steinbach, Sabine Schwarz, Helena E. Anheyer-Behmenburg, Astrid Sutor, Annette Schliephake, Diana Jeschke, Michael Striese, Franz Müller, Elisabeth Meyer-Kayser, Michael Stubbe, Natalia Osten-Sacken, Susann Krüger, Wolfgang Gaede, Martin Runge, Lothar Hoffmann, Hermann Ansorge, Franz J. Conraths, and Alain C. Frantz
Author affiliations: Musée National d'Histoire Naturelle, Luxembourg, Luxembourg (M. Heddergott, P. Steinbach, A.C. Frantz); Georg-August University, Göttingen, Germany (P. Steinbach); Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (S. Schwarz, A. Sutor, F.J. Conraths); Lower Saxony State Office for Consumer Protection and Food Safety, Hannover, Germany (H.E. Anheyer-Behmenburg, M. Runge); State Office for Consumer Protection Saxony-Anhalt, Stendal, Germany (A. Schliephake, W. Gaede); Senckenberg Museum of Natural History Görlitz, Görlitz, Germany (D. Jeschke, M. Striese, H. Ansorge); Justus-Liebig-University Giessen, Giessen, Germany (F. Müller); Thuringia Office for Consumer Protection, Bad Langensalza, Germany (E. Meyer-Kayser, L. Hoffmann); Martin-Luther University Halle-Wittenberg, Halle/Saale, Germany (M. Stubbe); Nicolaus Copernicus University, Toruń, Poland (N. Osten-Sacken); Fondation Faune-Flore, Luxembourg (N. Osten-Sacken); German Hunting Association, Berlin, Germany (A. Sutor, S. Krüger); International Institute Zittau, Technische Universität Dresden, Zittau, Germany (H. Ansorge)

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Abstract

Infestation with Baylisascaris procyonis, a gastrointestinal nematode of the raccoon, can cause fatal disease in humans. We found that the parasite is widespread in central Germany and can pose a public health risk. The spread of B. procyonis roundworms into nematode-free raccoon populations needs to be monitored.

The raccoon roundworm (Baylisascaris procyonis) is a gastrointestinal parasitic nematode of the raccoon (Procyon lotor). It is common in its native range in North America, where its prevalence in raccoons can reach 82% (1). Through their feces, infested raccoons can shed millions of B. procyonis eggs, which may remain infective in the environment for years (2). Paratenic hosts can acquire the parasite when ingesting nematode eggs from raccoon latrines (3).

B. procyonis infestations are usually benign in the raccoon but can be fatal in paratenic hosts, including humans (1). Since 1980, several fatal cases of neural larva migrans have occurred in humans in the United States (3); infants have been frequently affected because of fecal–oral transmission (4). Increasing raccoon densities in close proximity to humans has increased public health concern about B. procyonis roundworms (2).

As a result of joint translocation with raccoons, B. procyonis roundworms have increased their geographic range (5). Raccoons are common in Germany and Luxembourg (Figure, panel A). All raccoons in Germany are assumed to have stemmed from a small number of founders and 2 separate introduction events in western Germany (Hesse) during the 1930s and eastern Germany (Brandenburg) during the 1940s (8). However, genetic analysis has inferred a minimum of 5 founder events (6). In addition to 2 genetic populations clustered around the known introduction sites (referred to as the Hesse and Brandenburg populations), distinct raccoon populations were identified in Saxony (eastern Germany), around the Harz Mountains in central Germany, and in Luxembourg and neighboring regions (Figure, panel B).

B. procyonis roundworms occur in the Hesse and Harz populations (5) but are absent from Brandenburg (9). No information is available about the remaining 2 populations in Luxembourg and Saxony. Although only a few human cases of baylisascariasis have been reported from Germany (9), a detailed overview of the parasite’s geographic distribution is needed to identify potential risk areas.

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Thumbnail of Characteristics of the geographic distribution of the raccoon roundworm (Baylisascaris procyonis). A) Geographic origin of 8,184 dissected raccoons and the German administrative districts (Landkreise) in which raccoons were harvested during 2017–2018. Dots indicate sampling sites. B) Population genetic structure of raccoons in Germany and Luxembourg. Reanalysis of the dataset by (5) but including 26 raccoons from Luxembourg (genotyped following [5]) and omitting animals from the cit

Figure. Characteristics of the geographic distribution of the raccoon roundworm (Baylisascaris procyonis). A) Geographic origin of 8,184 dissected raccoons and the German administrative districts (Landkreise) in which raccoons were harvested during 2017–2018....

During 2008–2018, we collected 8,184 legally harvested or road-killed raccoons from Germany and Luxembourg (Figure, panel A), focusing on different regions every year or every few years, and investigated their intestines for the presence of B. procyonis roundworms. We plotted the presence of the parasite onto the 10 × 10–km ETRS89-LAEA5210 EEA reference grid, a base map provided by the European Environment Agency (https://www.eea.europa.eu/data-and-maps/data/eea-reference-grids-2). We calculated the proportion of infested raccoons for 69 of Germany’s 294 administrative districts where B. procyonis roundworms were present and >25 raccoons had been sampled. We generated maps by using ArcMap v.10.3 (ESRI Inc. https://www.esri.com).

B. procyonis roundworms were widespread in central Germany, their distribution probably corresponding to the geographic extent of the Hesse and Harz genetic populations (Figure, panels B, C). However, we did not detect the parasite in Luxembourg and western areas of Germany or in a northern/eastern region that included the federal states of Brandenburg, Mecklenburg-Western Pomerania, Schleswig-Holstein, northern parts of Lower Saxony, Saxony-Anhalt, and eastern Saxony. In other words, the parasite was not detected in the areas covered by the Luxembourg, Brandenburg, and Saxony genetic populations (Figure, panels B, C). A median of 43.6% (interquartile range 34.4%–49.7%) of raccoons were infested in the 69 administrative districts where the parasite was present and >25 raccoons had been sampled.

Identification of risk areas for human B. procyonis roundworm infestation is necessary because of the frequent proximity of raccoons to human populations. Our results show that the nematode is widespread and prevalent in central Germany. Given that B. procyonis eggs remain infective for years, the nematode is likely to pose a public health risk in its distribution area (10). To reduce the risk for B. procyonis infestation, protective measures (procedure masks, gloves, handwashing) should always be applied when raccoons or their feces in the risk area are handled. In this context, educational material should be made available to schools and daycare centers and to persons who have occupational contact with raccoons.

The match between the distribution of the roundworm and the extent of the different genetic populations of the raccoon suggests that the absence of the parasite results from the founder animals’ parasite-free status. However, we cannot exclude the possibility that ecologic or geographic differences between the introduction sites also contributed to the lack of parasites in some populations. Further research and monitoring are needed, especially in view of a possible spread of the parasite into nematode-free raccoon populations. Also, because of the rapid spread of raccoons, assessment of the status of the parasite in northwestern and southwestern Germany and at the periphery of its current distribution more generally should be considered.

Mr. Heddergott is scientific collaborator of the Musée National d’Historie Naturelle, Luxembourg. His primary research interest is mammalian parasites.

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Acknowledgment

We thank all regional and district hunting associations who provided raccoons for the study. We thank Louise Nicht for her help with dissection work.

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References

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DOI: 10.3201/eid2604.191670

Original Publication Date: March 05, 2020

Table of Contents – Volume 26, Number 4—April 2020

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Mike Heddergott, Musée National d'Histoire Naturelle, 25 Rue Münster, 2160 Luxembourg, Luxemb

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Page created: March 17, 2020
Page updated: March 17, 2020
Page reviewed: March 17, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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