Volume 26, Number 8—August 2020
Dispatch
Plasma-Derived Extracellular Vesicles as Potential Biomarkers in Heart Transplant Patient with Chronic Chagas Disease
Figure 1
![Isolation and characterization of plasma-derived EVs. A) Schematic diagram of the isolation and characterization of EVs derived from plasma samples. The details of each step are explained in The Study section. B) EVs were characterized by BBA using the classical EV markers CD5L, CD9, and CD63. C) NTA of SEC fractions F7–10. BBA, bead-based assay; EV, extracellular vesicle; LC-MS/MS, liquid chromatography–tandem mass spectrometry; MFI, median fluorescence intensity; NTA, nanoparticle tracking ana](/eid/images/19-1042-F1.jpg)
Figure 1. Isolation and characterization of plasma-derived EVs. A) Schematic diagram of the isolation and characterization of EVs derived from plasma samples. The details of each step are explained in The Study section. B) EVs were characterized by BBA using the classical EV markers CD5L, CD9, and CD63. C) NTA of SEC fractions F7–10. BBA, bead-based assay; EV, extracellular vesicle; LC-MS/MS, liquid chromatography–tandem mass spectrometry; MFI, median fluorescence intensity; NTA, nanoparticle tracking analysis; SEC, size-exclusion chromatography.
1Current affiliation: CIBER Epidemiología y Salud Pública (CIBERESP); Centro Nacional de Epidemiología, Instituto de Salud Carlos III, Madrid, Spain.
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