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Volume 27, Number 4—April 2021

Venezuelan Equine Encephalitis Complex Alphavirus in Bats, French Guiana

Carlo Fischer1, Dominique Pontier1, Ondine Filippi-Codaccioni, Jean-Batiste Pons, Ignacio Postigo-Hidalgo, Jeanne Duhayer, Sebastian Brünink, and Jan Felix DrexlerComments to Author 
Author affiliations: Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Institute of Virology, Berlin, Germany (C. Fischer, I. Postigo-Hidalgo, S. Brünink, J.F. Drexler); Université de Lyon, Villeurbanne, France (D. Pontier, O. Filippi-Codaccioni, J.-B. Pons, J. Duhayer); German Centre for Infection Research, Berlin, Germany (J.F. Drexler)

Main Article

Table 2

Oligonucleotides for quantification of TONV, French Guiana*

Name Sequence, 5′ → 3′ Concentration

*We carried out 25 μL real-time RT-PCR reactions using the Superscript III one-step RT-PCR system with Platinum Taq polymerase (Thermo Fisher Scientific, Reactions were set up with 5 μL extracted RNA; 12.5 μL of 2× reaction buffer; 0.4 μL of a 50 mM magnesium sulfate solution; 1 μg of nonacetylated bovine serum albumin; and 1 μL enzyme. Amplification was conducted at 50°C for 15 min, followed by 95°C for 3 min and 45 cycles of 95°C for 15 s and 58°C for 30 s with fluorescence, read at the 58° annealing/extension step on a LightCycler 480 thermocycler (Roche, FAM, fluorescein amidite; RT-PCR, reverse transcription PCR.

Main Article

1These authors contributed equally to this article.

Page created: January 21, 2021
Page updated: April 06, 2021
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