Figure. Maximum-likelihood tree of TULV from an immunocompetent patient in Germany (strain H18045/Winsen/19, marked with an asterisk [*]). Tree is based on partial large segment sequences (nucleotide position 2996–3291, according to TULV strain Moravia [GenBank accession no. NC_005226.1]). Designations of patient-derived sequences are shaded in gray. The alignment was constructed using the ClustalW Multiple Alignment algorithm implemented in Bioedit 7.2.3 (https://bioedit.software.informer.com). Maximum-likelihood analyses with 1,000 bootstraps and 50% cutoff using the general time-reversible substitution model with invariant sites and a gamma-distributed shape parameter was performed using FastTreeMP 2.1.10 (http://www.microbesonline.org) on CIPRES Science Gateway 3.3 (http://www.phylo.org). Bootstrap values >75 are given at the supported nodes. Geographic origin of TULV sequences are indicated by countries (Germany, DE; Czech Republic, CZ; France, FR; Poland, PL) and specified for Germany by adding the federal states (BE, Berlin; BB, Brandenburg; BW, Baden-Wuerttemberg, BY, Bavaria; MV, Mecklenburg-Western Pomerania, NI, Lower Saxony; NW, North Rhine-Westphalia; SH, Schleswig-Holstein; SN, Saxony; and TH, Thuringia). Triangles indicate condensed branches of TULV clades central north (CEN.N; DE: BB, GenBank accession nos. MK53017, MK53034, MK53036; BE, MK53003; MV, MK53004, MK53022; NI, MK53011, MK53032; SH, MK53033; SN HQ728453, HQ728454; TH, HQ728456, HQ728461, MK53007), eastern north (EST.N; PL: MK535037; DE: BB, MK535014–MK535015), eastern south (EST.S; CZ: MK386155–MK386156; DE: BY, MK386154, MK386161, MK386164), and central south (CEN.S; DE: BW, HQ728457, HQ728458; BY, HQ728462–HQ728464, HQ728466), as well as Puumala virus (KJ994778, MN026167, MN026168), Tatenale virus including its strain Traemmersee virus (MK542664, MK883760, MK883761, MN267824), Dobrava–Belgrade virus (JQ026206, KJ182937, KJ182938), Asikkala virus (KC880348, KC880349), Seewis virus (JQ425312, JQ425320), and Seoul virus (MG386252, KJ502300, KJ502303).