Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 27, Number 6—June 2021

Pertactin-Deficient Bordetella pertussis, Vaccine-Driven Evolution, and Reemergence of Pertussis

Longhuan Ma1, Amanda Caulfield1, Kalyan K. DewanComments to Author , and Eric T. Harvill
Author affiliation: University of Georgia College of Veterinary Medicine, Athens, Georgia, USA

Cite This Article


Recent reemergence of pertussis (whooping cough) in highly vaccinated populations and rapid expansion of Bordetella pertussis strains lacking pertactin (PRN), a common acellular vaccine antigen, have raised the specter of vaccine-driven evolution and potential return of what was once the major killer of children. The discovery that most circulating B. pertussis strains in the United States have acquired new and independent disruptive mutations in PRN is compelling evidence of strong selective pressure. However, the other 4 antigens included in acellular vaccines do not appear to be selected against so rapidly. We consider 3 aspects of PRN that distinguish it from other vaccine antigens, which might, individually or collectively, explain why only this antigen is being precipitously eliminated. An understanding of the increase in PRN-deficient strains should provide useful information for the current search for new protective antigens and provide broader lessons for the design of improved subunit vaccines.

Bordetella pertussis, the causative agent of pertussis (whooping cough), continues to reemerge in countries that have high vaccine coverage, such as the United States, and has accelerated since the switch during the mid-1990s from whole-cell pertussis (wP) formulations comprising many partially characterized bacterial proteins to the less reactogenic 1–5 component acellular pertussis (aP) vaccines (1,2). These aP vaccines, including DTaP (diphtheria, tetanus, and aP for children) and Tdap (tetanus, diphtheria, and aP for adolescents and adults), protect against disease, but this protection wanes rapidly and does not prevent colonization or transmission of the pathogen (35). In this background of suboptimally performing aP vaccines, many countries have noted the emergence and expansion of strains specifically lacking pertactin (PRN), a membrane bound autotransporter, and 1 of up to 5 B. pertussis protein antigens included in the vaccines (611).

PRN-deficient B. pertussis strains have recently been reported in countries using aP vaccines, including the United States, Australia, Sweden, Italy, Norway, the United Kingdom, France, Belgium, Finland, the Netherlands, and Japan. The frequency of PRN-deficient strains has been variable, but these strains have risen to dominance in the United States (85%), Australia (>80%), Sweden (69%), and Italy (55%) (711). Lower frequencies were reported from Japan, which showed a major decrease from a prevalence of 41% during 2008–2010 to 8% during 2014–2016 and correlated with a change to aP vaccine formulations that exclude PRN (6,1112). Denmark, which uses the monocomponent pertussis toxin (PT) vaccine, had no reports of PRN-deficient isolates before 2012, and the 4 PRN-deficient strains detected since have been associated with human migration from countries with PRN in their vaccines (6). Limited data are available from the predominantly developing countries that use wP vaccines to enable a robust comparison between the effects that aP and wP have on the selection of PRN. A sequencing study of the only 2 clinical isolates reported from India, which still uses wP, showed that the isolates still retained the broadly encountered PRN gene allele prn-1 (13). Considered together, these observations provide a strong correlation between the use of aP vaccines containing PRN and the appearance and increase to prominence of PRN-deficient strains.

Lineages of all bacteria are constantly evolving, but increasing to dominance alone is not conclusive evidence of a causal relationship between use of PRN-containing aP vaccines and loss of PRN. A PRN mutation could be carried along with a strain that is increasing in dominance because of 1 or many other mutations. However, the appearance of a wide variety of PRN mutations, each arising from a diversity of B. pertussis lineages over time, provides additional strong evidence in favor of vaccine-driven selection on PRN in particular. Although insertions of IS481 at multiple genomic locations are the most common PRN mutation, there is a large diversity of disruptions to PRN expression, including deletions within the signal sequence, promoter inversion, transversions resulting in a stop codon, deletions resulting in a stop codon, and full-gene deletion (2,6,12). The variety of genomic lesions that have led to loss of PRN indicates that numerous independent selection and expansion events have occurred in most of the lineages now circulating in many countries using aP vaccines. Providing more direct experimental data, such as murine models aimed at investigating PRN-deficient B. pertussis infection, have demonstrated an overall defect in colonization in unvaccinated mice, but advantages in both colonization and competition in assays using aP-vaccinated mice (1416).

Together, these observations strongly support the hypothesis that loss of PRN confers a fitness advantage over wildtype B. pertussis particular to the aP vaccinated populations in which they are arising. However, there are 4 other antigens included in aP vaccines that are not being disrupted or lost. Why are the other vaccine antigens not being mutated at similar rates? What are the characteristics of PRN that might lead to the loss of this antigen in particular? Understanding multiple possible explanations, and distinguishing between them where possible, will be useful for ongoing efforts to improve vaccines to control B. pertussis spread and disease.

Role of PRN

PRN is an autotransporter protein located on the surface of B. pertussis (17). Similar to all autotransporters found in gram-negative pathogens, PRN has 3 functional domains: the N terminal signal sequence, the passenger domain, and a C-terminal autotransporter domain. The signal sequence guides the passenger and transporter domains into the periplasm, enabling the transporter domain to form a pore in the outer membrane for translocation of the passenger domain to the cell surface. The protein is then cleaved by an outer membrane protease, the passenger domain remaining in contact with the surface by noncovalent interactions (18,19).

The function of PRN is only partially understood. PRN is considered one of several virulence factors found in B. pertussis and has been shown to serve as an adhesin, facilitating attachment to various mammalian epithelial cells (20,21). The 3-dimensional structure of PRN (PDB no. 1DAB) shows 16 right-handed parallel β-helixes, the largest β-helix structure recorded to date. Two Arg–Gly–Asp tripeptide motifs within the helical structure appear to be potential attachment sites to many mammalian adhesion proteins (2224). PRN is reported to be essential for resisting neutrophil-mediated clearance and possesses additional immunomodulatory abilities that aid B. pertussis in suppressing the production of proinflammatory cytokines (25,26). The benefits of functional expression of PRN in pathogenesis are consistent with its conservation in B. pertussis and other pathogenic Bordetella species. Loss of such a factor would be expected to be costly to the organism, yet PRN-deficient strains appear to be rapidly expanding in aP vaccinated populations, suggesting a recent rebalancing of fitness costs and benefits.

Unique Features of PRN among Vaccine Antigens

PRN is conserved in B. pertussis, B. parapertussis, and B. bronchiseptica under the same Bordetella virulence gene regulatory system, suggesting that PRN has a more general role in pathogenesis that is not restricted to the human-specific B. pertussis. However, there are 16 other autotransporter genes identified in the genome of B. pertussis, 5 of which are disrupted by frameshift mutations (27). Park et al. (28) observed that autotransporters, as a group, are much more highly mutated or lost than other virulence factors. There appears to be no major loss in in vitro growth or in vivo fitness that prevents the expansion of B. pertussis strains lacking PRN, indicating that the functional contributions of PRN in pathogenesis might be redundant or that complementary functions, potentially mediated by other autotransporters, might be compensating for any deficiencies caused its loss. This finding can be contrasted with PT, which requires a complex operon to assemble and another to export, has a central and nonredundant role in the pathogenesis of B. pertussis, and has no paralogs in the genome that can replace it. This finding is consistent with the rarity of clinical isolates lacking PT, compared with the abundance of PRN-deficient strains (2931). For these and potentially other reasons, it might be that loss of PRN can be tolerated, but loss of PT would result in a more serious fitness defect.

Figure 1

Model for various roles of antibodies against antigens in acellular pertussis vaccine. A) Antibodies against PT and FHA neutralize secreted virulence factors and mitigate disease progression but are not targeted to the bacterial surface. B) Antibodies attaching to fimbriae poorly activate the complement system far from the bacterial membrane. C) Antibody-PRN complex induces strong bactericidal activity via multiple synergistic functions. This complex activates complement to form a MAC, activates complement to deposit components such as C3b that opsonize the bacterial surface, and binds FcRs on phagocytes to activate phagocytosis. PRN labels indicate strains specifically lacking PRN. Bp, Bordetella pertussis; CR1, complement receptor type 1; FcR, fragment crystallizable region; FHA, filamentous hemagglutinin; MAC, membrane attack complex; PRN, pertactin; PT, pertussis toxin.

Figure 1. Model for various roles of antibodies against antigens in acellular pertussis vaccine. A) Antibodies against PT and FHA neutralize secreted virulence factors and mitigate disease progression but are not targeted...

Examination of the location and conformation of the different acellular vaccine components showed that most antibody functions directed against these antigens occur away from the bacterial surface. Both PT and the mature surface-associated filamentous hemagglutinin (FHA) (32) are secreted and diffuse away into the surrounding host environment. Therefore, antibodies directed against released FHA and PT would primarily neutralize toxin function by binding to the secreted molecules in the surrounding milieu, or to molecules in the process of being shed, mitigating potential surface-directed antibody effects. These antibodies do not effectively localize to the bacterial surface and thus do not facilitate complement activation or fragment crystallizable region (FcR)–mediated phagocytosis that would result in direct bacterial killing (Figure 1, panel A) (33,34). Fimbriae (FIM), although generally anchored to the bacteria, might extend more than a cell length away from the cell surface. Because FIM are composed of repeated structures with many copies of the same antigenic molecule, most FIM antibodies would be bound to parts of the structure extended >1 micron from the bacterial membrane, which is an enormous distance for highly reactive complement components to travel. Antibodies bound to fimbria would not be arrayed on a 2-dimensional surface required to optimally bind and activate complement or FcRs (Figure 1, panel B) (35).

However, PRN is the only aP vaccine antigen that remains closely associated with the outer membrane. When cognate antibodies bind PRN, they become arrayed on the bacterial surface in a conformation that is particularly effective in binding and activating complement component C1q. The subsequent complement cascade that is activated rapidly deposits component in the adjacent membrane that opsonizes the cell and assembles into a membrane attack complex to lyse the bacterial membrane (3336). The combination of the array of antibodies, bolstered by the complement components cleaved and activated in the immediate vicinity, would effectively opsonize the bacteria for efficient phagocytic killing (Figure 1, panel C).

The model we provide (Figure 1), although largely hypothetical, is consistent with evidence that aP vaccination is effective in preventing severe disease (by binding and neutralizing a key factor that mediate aspects of disease) but is much less effective in preventing nasopharyngeal colonization. Most antibodies, similar to those directed against secreted PT/FHA or distal FIM, do not effectively target and kill the bacteria. This model would also explain why loss of PRN might enable partial evasion of aP-induced immunity, and is also consistent with human surveys that showed that 3-component aP vaccines containing PT, FHA, and PRN were more efficacious than 2-component vaccines lacking PRN (37,38).

Persistence of PRN Antibodies

Figure 2

Differential decay of antibodies against acellular pertussis vaccine antigens and their effective capacity for protection. Antibodies against PRN and FHA remain at relatively higher titers for a longer period. However, PT-specific antibodies decrease to low titers rapidly. A consistently low level of antibodies against FIM is induced. Solid lines indicate antibodies that have high protective capacity, and dotted lines indicate antibodies that had low protective capacity. Only PRN antibodies are highly protective and persist at high titers for years. FHA, filamentous hemagglutinin, FIM, fimbriae; PT, pertussis toxin; PRN, pertactin.

Figure 2. Differential decay of antibodies against acellular pertussis vaccine antigens and their effective capacity for protection. Antibodies against PRN and FHA remain at relatively higher titers for a longer period. However,...

Despite the shortcomings of aP vaccines in generating a strong memory response, and the resulting waning immunity of these vaccines against disease (39,40), aP vaccines induce robust IgG titers against most of its component antigens. However, this strong humoral response, although protecting against symptoms of pertussis, does not prevent the pathogen from colonizing the upper respiratory tract or from transmitting between hosts (3). Furthermore, although initially induced at high levels, circulating antibodies decay relatively rapidly across all age groups (4,4043). Studies evaluating dynamic levels of specific antibodies across time have consistently show differential rates of decay for the various antigen-specific antibodies, with PT antibody titers decaying more rapidly than antibodies to FHA and PRN (Figure 2). Similarly, FIM2/3 have been reported to poorly stimulate generation of protective antibodies postinfection and postvaccinations (4,43), although these data conflict with those of other reports that shown higher levels of FIM antibodies, even at 10 years postvaccination. However, the higher antibody titers observed in these reports are also noted to decrease sharply and are likely to reach unprotective levels over a relatively short time. This rapid waning immunity against PT and FIM would be expected to narrow the window of selective pressure against these antigens.

In contrast to antibodies against PT, antibodies against PRN and FHA are relatively more persistent (Figure 2) (38,44,45), suggesting that there is a longer period after vaccination when there are effective titers of antibodies against FHA and PRN. This finding would be expected to result in strong pressure against PRN and FHA. However, in a search for serologic correlates of immunity to pertussis, Le et al. (4) noted that FHA provides relatively little contribution to protection but PRN had a higher protective role.

These observations have recently been validated in studies by Lesne et al., who used human serum bactericidal assays to determine that antibodies to PRN, but no other aP component, are bactericidal in in vitro complement killing assays (46). These findings somewhat conflict with those of previous studies and testing methods, which often prioritize PT IgG as an indicator for protection against pertussis (4,45,47). However, the short period during which levels of neutralizing antibodies against PT remain elevated, in contrast to bactericidal antibodies against PRN, suggests that PRN antibodies might be a more appropriate measure for pertussis immunity.

The short period during which antibodies to PT remain at elevated levels indicates that there is a longer period when antibodies to PRN remain at high levels, but levels of antibodies to PT have decreased. Le et al. also noted that a much larger proportion of enrolled patients tested before aP vaccination already had high titers of antibodies to PRN, and many had antibodies to PT (4). Therefore, in addition to its surface localization and strong opsonizing potential, the persistence of the PRN antibodies is likely to contribute to prolonged selection against this antigen in particular.


The rapid reemergence of pertussis noted in the early 2000s brought much initial speculation. Factors such as human migration, increased sensitivity of testing, increased volumes of testing, and reporting through heightened surveillance have been proposed to contribute to the observed resurgence (13). However, the collective experience in countries switching from wP vaccines to aP vaccines strongly suggests that these safer, but less effective, vaccines have contributed to the resurgence of pertussis. In addition, the way multiple PRN-deficient strains have swept across countries that use aP vaccines presents a strong case in favor of vaccine-driven selection against PRN (612). What has remained unclear is what will be the consequences of these changes. Will PRN-deficient strains continue to evolve, loosing other vaccine antigens until they escape vaccine effects completely? Or will the loss of these factors result in strains attenuated in virulence such that they become more like commensals than pathogens? Or is PRN really the only antigen that can be lost without serious fitness costs?

The 3 aspects of PRN we have highlighted for selective loss of PRN (i.e., its functional redundancy, the relatively longer functional persistence of antibodies against it, and its close location to the surface membrane for productive complement fixation) are not an exhaustive list of all possibilities and are not mutually exclusive. Sufficient sustained selective pressure against any particular antigen is likely to lead to its loss or change. Efforts to improve the current vaccine should be informed by a careful consideration of the lessons learned from this instance. If PRN is being lost because it is the only surface antigen, then should we replace it with 1 or more new surface antigens? If it is lost because its function is at least partly redundant then should we select some molecule(s) that is less likely to be redundant? In designing new vaccines, it would be prudent to carefully consider the issues that appear to be enabling potential vaccine escape mutants, such as PRN-deficient strains, to rapidly expand and rise to prominence.

Mr. Longhuan and Ms. Caulfield are graduate students in the Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA. Their primary research interests are host–pathogen interactions and the transmission of B. pertussis by using mouse models of infection.



This study was supported by the National Science Foundation (grant no. DGE-1545433) and the National Institutes of Health (grant nos. R21DC018496, 1R21AI156293-01, 1R21AI159347-01, and 1R56AI149787-01A1).



  1. Jackson  DW, Rohani  P. Perplexities of pertussis: recent global epidemiological trends and their potential causes. Epidemiol Infect. 2014;142:67284. DOIPubMedGoogle Scholar
  2. Pawloski  LC, Queenan  AM, Cassiday  PK, Lynch  AS, Harrison  MJ, Shang  W, et al. Prevalence and molecular characterization of pertactin-deficient Bordetella pertussis in the United States. Clin Vaccine Immunol. 2014;21:11925. DOIPubMedGoogle Scholar
  3. Warfel  JM, Zimmerman  LI, Merkel  TJ. Acellular pertussis vaccines protect against disease but fail to prevent infection and transmission in a nonhuman primate model. Proc Natl Acad Sci U S A. 2014;111:78792. DOIPubMedGoogle Scholar
  4. Le  T, Cherry  JD, Chang  SJ, Knoll  MD, Lee  ML, Barenkamp  S, et al.; APERT Study. Immune responses and antibody decay after immunization of adolescents and adults with an acellular pertussis vaccine: the APERT Study. J Infect Dis. 2004;190:53544. DOIPubMedGoogle Scholar
  5. Breakwell  L, Kelso  P, Finley  C, Schoenfeld  S, Goode  B, Misegades  LK, et al. Pertussis vaccine effectiveness in the setting of pertactin-deficient pertussis. Pediatrics. 2016;137:e20153973. DOIPubMedGoogle Scholar
  6. Barkoff  AM, Mertsola  J, Pierard  D, Dalby  T, Hoegh  SV, Guillot  S, et al. Pertactin-deficient Bordetella pertussis isolates: evidence of increased circulation in Europe, 1998 to 2015. Euro Surveill. 2019;24:1700832. DOIPubMedGoogle Scholar
  7. Martin  SW, Pawloski  L, Williams  M, Weening  K, DeBolt  C, Qin  X, et al. Pertactin-negative Bordetella pertussis strains: evidence for a possible selective advantage. Clin Infect Dis. 2015;60:2237. DOIPubMedGoogle Scholar
  8. Byrne  S, Slack  AT. Analysis of Bordetella pertussis pertactin and pertussis toxin types from Queensland, Australia, 1999-2003. BMC Infect Dis. 2006;6:53. DOIPubMedGoogle Scholar
  9. Weigand  MR, Williams  MM, Peng  Y, Kania  D, Pawloski  LC, Tondella  ML; CDC Pertussis Working Group. Genomic survey of Bordetella pertussis diversity, United States, 2000–2013. Emerg Infect Dis. 2019;25:7803. DOIPubMedGoogle Scholar
  10. Hiramatsu  Y, Miyaji  Y, Otsuka  N, Arakawa  Y, Shibayama  K, Kamachi  K. Significant decrease in pertactin-deficient Bordetella pertussis isolates, Japan. Emerg Infect Dis. 2017;23:699701. DOIPubMedGoogle Scholar
  11. Zomer  A, Otsuka  N, Hiramatsu  Y, Kamachi  K, Nishimura  N, Ozaki  T, et al. Bordetella pertussis population dynamics and phylogeny in Japan after adoption of acellular pertussis vaccines. Microb Genom. 2018;4:e000180. DOIPubMedGoogle Scholar
  12. Otsuka  N, Han  HJ, Toyoizumi-Ajisaka  H, Nakamura  Y, Arakawa  Y, Shibayama  K, et al. Prevalence and genetic characterization of pertactin-deficient Bordetella pertussis in Japan. PLoS One. 2012;7:e31985. DOIPubMedGoogle Scholar
  13. Alai  S, Ghattargi  VC, Gautam  M, Patel  K, Pawar  SP, Dhotre  DP, et al. Comparative genomics of whole-cell pertussis vaccine strains from India. BMC Genomics. 2020;21:345. DOIPubMedGoogle Scholar
  14. van Gent  M, van Loo  IH, Heuvelman  KJ, de Neeling  AJ, Teunis  P, Mooi  FR. Studies on Prn variation in the mouse model and comparison with epidemiological data. PLoS One. 2011;6:e18014. DOIPubMedGoogle Scholar
  15. Safarchi  A, Octavia  S, Luu  LD, Tay  CY, Sintchenko  V, Wood  N, et al. Pertactin negative Bordetella pertussis demonstrates higher fitness under vaccine selection pressure in a mixed infection model. Vaccine. 2015;33:627781. DOIPubMedGoogle Scholar
  16. Hegerle  N, Dore  G, Guiso  N. Pertactin deficient Bordetella pertussis present a better fitness in mice immunized with an acellular pertussis vaccine. Vaccine. 2014;32:6597600. DOIPubMedGoogle Scholar
  17. Brennan  MJ, Li  ZM, Cowell  JL, Bisher  ME, Steven  AC, Novotny  P, et al. Identification of a 69-kilodalton nonfimbrial protein as an agglutinogen of Bordetella pertussis. Infect Immun. 1988;56:318995. DOIPubMedGoogle Scholar
  18. Henderson  IR, Navarro-Garcia  F, Nataro  JP. The great escape: structure and function of the autotransporter proteins. Trends Microbiol. 1998;6:3708. DOIPubMedGoogle Scholar
  19. Wells  TJ, Tree  JJ, Ulett  GC, Schembri  MA. Autotransporter proteins: novel targets at the bacterial cell surface. FEMS Microbiol Lett. 2007;274:16372. DOIPubMedGoogle Scholar
  20. Everest  P, Li  J, Douce  G, Charles  I, De Azavedo  J, Chatfield  S, et al. Role of the Bordetella pertussis P.69/pertactin protein and the P.69/pertactin RGD motif in the adherence to and invasion of mammalian cells. Microbiology (Reading). 1996;142:32618. DOIPubMedGoogle Scholar
  21. Leininger  E, Roberts  M, Kenimer  JG, Charles  IG, Fairweather  N, Novotny  P, et al. Pertactin, an Arg-Gly-Asp-containing Bordetella pertussis surface protein that promotes adherence of mammalian cells. Proc Natl Acad Sci U S A. 1991;88:3459. DOIPubMedGoogle Scholar
  22. Emsley  P, McDermott  G, Charles  IG, Fairweather  NF, Isaacs  NW. Crystallographic characterization of pertactin, a membrane-associated protein from Bordetella pertussis. J Mol Biol. 1994;235:7723. DOIPubMedGoogle Scholar
  23. Emsley  P, Charles  IG, Fairweather  NF, Isaacs  NW. Structure of Bordetella pertussis virulence factor P.69 pertactin. Nature. 1996;381:902. DOIPubMedGoogle Scholar
  24. Junker  M, Schuster  CC, McDonnell  AV, Sorg  KA, Finn  MC, Berger  B, et al. Pertactin β-helix folding mechanism suggests common themes for the secretion and folding of autotransporter proteins. Proc Natl Acad Sci U S A. 2006;103:491823. DOIPubMedGoogle Scholar
  25. Inatsuka  CS, Xu  Q, Vujkovic-Cvijin  I, Wong  S, Stibitz  S, Miller  JF, et al. Pertactin is required for Bordetella species to resist neutrophil-mediated clearance. Infect Immun. 2010;78:29019. DOIPubMedGoogle Scholar
  26. Hovingh  ES, Mariman  R, Solans  L, Hijdra  D, Hamstra  HJ, Jongerius  I, et al. Bordetella pertussis pertactin knock-out strains reveal immunomodulatory properties of this virulence factor. Emerg Microbes Infect. 2018;7:39. DOIPubMedGoogle Scholar
  27. Parkhill  J, Sebaihia  M, Preston  A, Murphy  LD, Thomson  N, Harris  DE, et al. Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. Nat Genet. 2003;35:3240. DOIPubMedGoogle Scholar
  28. Park  J, Zhang  Y, Chen  C, Dudley  EG, Harvill  ET. Diversity of secretion systems associated with virulence characteristics of the classical bordetellae. Microbiology (Reading). 2015;161:232840. DOIPubMedGoogle Scholar
  29. Linz  B, Ivanov  YV, Preston  A, Brinkac  L, Parkhill  J, Kim  M, et al. Acquisition and loss of virulence-associated factors during genome evolution and speciation in three clades of Bordetella species. BMC Genomics. 2016;17:767. DOIPubMedGoogle Scholar
  30. Bouchez  V, Hegerle  N, Strati  F, Njamkepo  E, Guiso  N. New data on vaccine antigen deficient Bordetella pertussis isolates. Vaccines (Basel). 2015;3:75170. DOIPubMedGoogle Scholar
  31. Bouchez  V, Brun  D, Cantinelli  T, Dore  G, Njamkepo  E, Guiso  N. First report and detailed characterization of B. pertussis isolates not expressing Pertussis Toxin or Pertactin. Vaccine. 2009;27:603441. DOIPubMedGoogle Scholar
  32. Nash  ZM, Cotter  PA. Bordetella filamentous hemagglutinin, a model for the two-partner secretion pathway. Microbiol Spectr. 2019;7:31928.PubMedGoogle Scholar
  33. Hellwig  SM, Rodriguez  ME, Berbers  GA, van de Winkel  JG, Mooi  FR. Crucial role of antibodies to pertactin in Bordetella pertussis immunity. J Infect Dis. 2003;188:73842. DOIPubMedGoogle Scholar
  34. Weiss  AA, Mobberley  PS, Fernandez  RC, Mink  CM. Characterization of human bactericidal antibodies to Bordetella pertussis. Infect Immun. 1999;67:142431. DOIPubMedGoogle Scholar
  35. Lambris  JD, Ricklin  D, Geisbrecht  BV. Complement evasion by human pathogens. Nat Rev Microbiol. 2008;6:13242. DOIPubMedGoogle Scholar
  36. Jongerius  I, Schuijt  TJ, Mooi  FR, Pinelli  E. Complement evasion by Bordetella pertussis: implications for improving current vaccines. J Mol Med (Berl). 2015;93:395402. DOIPubMedGoogle Scholar
  37. Greco  D, Salmaso  S, Mastrantonio  P, Giuliano  M, Tozzi  AE, Anemona  A, et al.; Progetto Pertosse Working Group. A controlled trial of two acellular vaccines and one whole-cell vaccine against pertussis. N Engl J Med. 1996;334:3418. DOIPubMedGoogle Scholar
  38. Gustafsson  L, Hallander  HO, Olin  P, Reizenstein  E, Storsaeter  J. A controlled trial of a two-component acellular, a five-component acellular, and a whole-cell pertussis vaccine. N Engl J Med. 1996;334:34955. DOIPubMedGoogle Scholar
  39. van Twillert  I, Han  WG, van Els  CA. Waning and aging of cellular immunity to Bordetella pertussis. Pathog Dis. 2015;73:ftv071. DOIPubMedGoogle Scholar
  40. Tomovici  A, Barreto  L, Zickler  P, Meekison  W, Noya  F, Voloshen  T, et al. Humoral immunity 10 years after booster immunization with an adolescent and adult formulation combined tetanus, diphtheria, and 5-component acellular pertussis vaccine. Vaccine. 2012;30:264753. DOIPubMedGoogle Scholar
  41. Taranger  J, Trollfors  B, Lagergård  T, Sundh  V, Bryla  DA, Schneerson  R, et al. Correlation between pertussis toxin IgG antibodies in postvaccination sera and subsequent protection against pertussis. J Infect Dis. 2000;181:10103. DOIPubMedGoogle Scholar
  42. Munoz  FM, Bond  NH, Maccato  M, Pinell  P, Hammill  HA, Swamy  GK, et al. Safety and immunogenicity of tetanus diphtheria and acellular pertussis (Tdap) immunization during pregnancy in mothers and infants: a randomized clinical trial. JAMA. 2014;311:17609. DOIPubMedGoogle Scholar
  43. Abu Raya  B, Srugo  I, Kessel  A, Peterman  M, Vaknin  A, Bamberger  E. The decline of pertussis-specific antibodies after tetanus, diphtheria, and acellular pertussis immunization in late pregnancy. J Infect Dis. 2015;212:186973. DOIPubMedGoogle Scholar
  44. Heininger  U, Cherry  JD, Stehr  K. Serologic response and antibody-titer decay in adults with pertussis. Clin Infect Dis. 2004;38:5914. DOIPubMedGoogle Scholar
  45. Storsaeter  J, Hallander  HO, Gustafsson  L, Olin  P. Levels of anti-pertussis antibodies related to protection after household exposure to Bordetella pertussis. Vaccine. 1998;16:190716. DOIPubMedGoogle Scholar
  46. Lesne  E, Cavell  BE, Freire-Martin  I, Persaud  R, Alexander  F, Taylor  S, et al. Acellular pertussis vaccines induce anti-pertactin bactericidal antibodies which drives the emergence of pertactin-negative strains. Front Microbiol. 2020;11:2108. DOIPubMedGoogle Scholar
  47. Lee  AD, Cassiday  PK, Pawloski  LC, Tatti  KM, Martin  MD, Briere  EC, et al.; Clinical Validation Study Group. Clinical evaluation and validation of laboratory methods for the diagnosis of Bordetella pertussis infection: Culture, polymerase chain reaction (PCR) and anti-pertussis toxin IgG serology (IgG-PT). PLoS One. 2018;13:e0195979. DOIPubMedGoogle Scholar




Cite This Article

DOI: 10.3201/eid2706.203850

Original Publication Date: April 29, 2021

1These authors contributed equally to this article.

Table of Contents – Volume 27, Number 6—June 2021

EID Search Options
presentation_01 Advanced Article Search – Search articles by author and/or keyword.
presentation_01 Articles by Country Search – Search articles by the topic country.
presentation_01 Article Type Search – Search articles by article type and issue.



Please use the form below to submit correspondence to the authors or contact them at the following address:

Kalyan K. Dewan, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA

Send To

10000 character(s) remaining.


Page created: March 05, 2021
Page updated: May 18, 2021
Page reviewed: May 18, 2021
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.