Figure 2. Immunofluorescent images from Vero E6 cells exposed to semen samples obtained at day 7 after symptom onset from volunteers from Réunion Island with dengue virus (DENV) infection in a study of virus clearance and effects on reproductive function. A) Seminal plasma (patient 6); B) whole semen cells (patient 3); C) cells from 40% fraction obtained after semen preparation (patient 6); D) DENV-1 infected Vero E6 cells (positive control) (multiplicity of infection 0.01 for 3 days); E) noninfected Vero E6 cells; F) Vero E6 cells inoculated with noninfected semen (negative controls). VeroE6 cells were inoculated with the semen fractions indicated from DENV-infected (A, B, C) or uninfected patients (E) and cultured for 7 days (first passage). Images were made after detection of DENV envelop protein (DENV-E) from a second passage on VeroE6 of culture supernatants collected after the first passage. Red indicates DENV-E, blue indicates DAPI staining. Scale bars indicate 20 μm.