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Volume 28, Number 7—July 2022
Dispatch

Use of Human Intestinal Enteroids to Evaluate Persistence of Infectious Human Norovirus in Seawater

Marion Desdouits1Comments to Author , David Polo12, Cecile Le Mennec, Sofia Strubbia3, Xi-Lei Zeng, Khalil Ettayebi, Robert L. Atmar, Mary K. Estes, and Françoise S. Le Guyader
Author affiliations: Institut Franҫais de Recherche pour l’Exploitation de la Mer, Nantes, France (M. Desdouits, D. Polo, C. Le Mennec, S. Strubbia, F.S. Le Guyader); Baylor College of Medicine, Houston, Texas, USA (X.-L. Zeng, K. Ettayebi, R.L. Atmar, M.K. Estes)

Main Article

Table 1

Characteristics of seawater samples used for 3 experiments using human intestinal enteroids to evaluate persistence of infectious human norovirus

Experiment 1 2 3
Collection date*
 2018 Sep 5
 2018 Oct 16
 2019 Apr 30
Physio-chemistry
Salinity, % 36.5 (35†) 35 33.3
Turbidity, NTU 0.67 7.50 1.14
pH 7.8 7.9 7.9
Total suspended solids, mg/L 4.0 3.0 1.0
Dissolved organic carbon, mg/L 2.3 1.6 2.1
Phosphate, mg/L 0.079 0.082 0.192
Nitrate, mg/L
 0.4
 0.3
 0.8
Microbiology
Total nonmarine bacteria/100 mL 100 >300 37
Total marine bacteria/100 mL >300 >300 >300
Escherichia coli/100 mL 0 0 0

*Coastal seawater samples were collected and sand-filtered at the same experimental shellfish farm at different dates, kept at 4°C, and used within 1 week of collection. NTU, Nephelometric Turbidity Units. †Salinity of seawater was adjusted to 35% using distilled water for experiment 1.

Main Article

1These first authors contributed equally to this article.

2Current affiliation: Centro de Investigaciones Biologicas–Facultade de Bioloxía & CRETUS, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.

3Current affiliation: SECALIM UMR 104 Oniris/Inrae, Nantes, France.

Page created: May 18, 2022
Page updated: June 18, 2022
Page reviewed: June 18, 2022
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