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Volume 29, Number 11—November 2023
Synopsis

Detection of Novel US Neisseria meningitidis Urethritis Clade Subtypes in Japan

Hideyuki TakahashiComments to Author , Masatomo Morita, Mitsuru Yasuda, Yuki Ohama, Yoshitomo Kobori, Munekado Kojima, Ken Shimuta, Yukihiro Akeda, and Makoto Ohnishi
Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (H. Takahashi, M. Morita, Y. Ohama, K. Shimuta, Y. Akeda, M. Ohnishi); Sapporo Medical University, Sapporo, Japan (M. Yasuda); Private Care Clinic Tokyo, Tokyo (Y. Kobori); Nagoya Urology Hospital, Aichi, Japan (M. Kojima)

Main Article

Figure 2

Organization of genes within the cps locus of Neisseria meningitidis isolates in study of detection of novel US N. meningitidis urethritis clade subtypes in Japan. N. meningitidis isolates from Japan (NIID835, NIID836, NIID838) and United States (US_NmUC) were compared with N. meningitidis strain FAM18 (GenBank accession no. AM421808). Open red arrows indicate the cssA, cssB, cssC, csc, and cssE genes in region A responsible for capsule synthesis and open blue arrows the ctrD, ctrC, ctrB, and ctrA genes (in that order) in region C responsible for capsule transport. Insertion sequence IS1301 is indicated. Open reading frames identical to NMC0044 (solid red), NMC0049 (gray), NMC0068 (yellow), NMC0071 (green), NMC0073 (pink), and NMC0075 (blue) in FAM18 are shown for each isolate. Partial deletion is indicated for the csc gene (csc′). The cps locus for US_NmUC had 2 configurations created by a ≈20-kb genome inversion between 2 IS1301 sequences (designated as A and B). Gene alignments in the region between the 2 IS1301 sequences have been omitted and are indicated by the dashed line. Although ctrD, ctrC, ctrB, and ctrA genes were shown to be proximal to dnaJ (12), contigs containing the dnaJ-rfbC, rfbA, and rfbB genes and the ctrD, ctrC, ctrB, and ctrA genes (shown on the left side of A and B), as well as 2 IS1301 and pykA genes (shown on the right side of A and B), were not connected by our analysis because of the absence of US_NmUC long-read sequences. Therefore, unidentified connections of the 2 contigs are indicated by a dotted line.

Figure 2. Organization of genes within the cps locus of Neisseria meningitidis isolates in study of detection of novel US N. meningitidis urethritis clade subtypes in Japan. N. meningitidis isolates from Japan (NIID835, NIID836, NIID838) and United States (US_NmUC) were compared with N. meningitidis strain FAM18 (GenBank accession no. AM421808). Open red arrows indicate the cssA, cssB, cssC, csc, and cssE genes in region A responsible for capsule synthesis and open blue arrows the ctrD, ctrC, ctrB, and ctrA genes (in that order) in region C responsible for capsule transport. Insertion sequence IS1301 is indicated. Open reading frames identical to NMC0044 (solid red), NMC0049 (gray), NMC0068 (yellow), NMC0071 (green), NMC0073 (pink), and NMC0075 (blue) in FAM18 are shown for each isolate. Partial deletion is indicated for the csc gene (csc′). The cps locus for US_NmUC had 2 configurations created by a ≈20-kb genome inversion between 2 IS1301 sequences (designated as A and B). Gene alignments in the region between the 2 IS1301 sequences have been omitted and are indicated by the dashed line. Although ctrD, ctrC, ctrB, and ctrA genes were shown to be proximal to dnaJ (12), contigs containing the dnaJ-rfbC, rfbA, and rfbB genes and the ctrD, ctrC, ctrB, and ctrA genes (shown on the left side of A and B), as well as 2 IS1301 and pykA genes (shown on the right side of A and B), were not connected by our analysis because of the absence of US_NmUC long-read sequences. Therefore, unidentified connections of the 2 contigs are indicated by a dotted line.

Main Article

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