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Volume 30, Number 3—March 2024
Research

Microsporidia (Encephalitozoon cuniculi) in Patients with Degenerative Hip and Knee Disease, Czech Republic

Bohumil SakComments to Author , Petra Gottliebová, Elka Nyčová, Nikola Holubová, Jana Fenclová, Marta Kicia, Żaneta Zajączkowska, and Martin Kváč
Author affiliations: Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic (B. Sak, N. Holubová, J. Fenclová, M. Kváč); Bulovka Hospital, Prague, Czech Republic (P. Gottliebová, E. Nyčová); University of South Bohemia, České Budějovice (J. Fenclová, M. Kváč); Wroclaw Medical University, Wroclaw, Poland (M. Kicia, Ż. Zajączkowska)

Main Article

Figure 3

Microscopic analysis of Encephalitozoon cuniculi spores isolated from immunocompetent patients in study of microsporidia in patients with degenerative hip and knee disease, Czech Republic. Samples were collected from patients who tested positive for Encephalitozoon DNA during May 2020–September 2021 at Bulovka Hospital in Prague. Visualization of E. cuniculi from sample of knee joint fluid from patient no. 29 (A) and hip joint fluid from patient no. 2 (B). Arrows indicate E. cuniculi spores stained with Calcofluor M2R (Sigma Aldrich, https://www.sigmaaldrich.com) and viewed after fluorescence excitation at 490 nm wavelength. Scale bars are 10 μm.

Figure 3. Microscopic analysis of Encephalitozoon cuniculi spores isolated from immunocompetent patients in study of microsporidia in patients with degenerative hip and knee disease, Czech Republic. Samples were collected from patients who tested positive for Encephalitozoon DNA during May 2020–September 2021 at Bulovka Hospital in Prague. Visualization of E. cuniculi from sample of knee joint fluid from patient no. 29 (A) and hip joint fluid from patient no. 2 (B). Arrows indicate E. cuniculi spores stained with Calcofluor M2R (Sigma Aldrich, https://www.sigmaaldrich.com) and viewed after fluorescence excitation at 490 nm wavelength. Scale bars are 10 μm.

Main Article

Page created: January 12, 2024
Page updated: February 22, 2024
Page reviewed: February 22, 2024
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