Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 6, Number 1—February 2000

Molecular Typing of Multidrug-Resistant Salmonella Blockley Outbreak Isolates from Greece

Panayotis T. Tassios*, Christos Chadjichristodoulou†, Maria Lambiri‡, Athina Kansouzidou-Kanakoudi§, Zannina Sarandopoulou*, Jenny Kourea-Kremastinou‡, Leonidas S. Tzouvelekis*, and Nicholas J. Legakis*Comments to Author 
Author affiliations: *University of Athens, Athens, Greece; †National Center for Surveillance and Intervention, Athens, Greece; ‡National School of Public Health, Athens, Greece; §Salmonella Reference Center for Macedonia and Thrace, Thessaloniki, Greece

Main Article


Resistant phenotypes for isolates of Salmonella Blockley in Greece

PFGEa subtype (n) Resistance phenotypeb (n) Time span Locationsc
A1 (1) AT (1) May 3, 1998 1
A2 (12) ATC (9), TCN (1), 1997; May 16- 6
AN (1), AT (1) Oct 13, 1998
A3 (3) ATC (1), ATN (1), Aug 24- 3
TCN (1) Oct 27, 1998
A4 (12) ATCN (8), ATN (2), Aug 17, 1998- 6
ATC (1), TCN (1) Feb 23, 1999
A5 (1) ATCN (1) Sep 29, 1998 1
A6 (1) AT (1) 1996 -
A7 (1) ATCN (1) 1997 -
A8 (2) ATC (1), AN (1) 1997; Aug 10, 1

aPFGE, pulsed-field gel electrophoresis.
bA, kanamycin and streptomycin; T, tetracycline; C, chloramphenicol; N, nalidixic acid.
cNumber of locations of isolation during the outbreak.

Main Article

  1. Fisher  IST. on behalf of Enter-net participants. Enter-net Quarterly Salmonella Report - 98/3 (1998 Jul-Sep). Available from: URL:
  2. Limawongpranee  S, Hayashidani  H, Okatani  AT, Ono  K, Hirota  C, Kaneko  K, Prevalence and persistence of Salmonella in broiler chicken flocks. J Vet Med Sci. 1999;61:2559. DOIPubMedGoogle Scholar
  3. Matsushita  S, Yamada  S, Sekiguchi  K, Kusunokui  J, Ohta  K, Kudoh  Y. Serovar-distribution and drug-resistance of Salmonella strains isolated from domestic and imported cases in 1990-1994 in Tokyo. Kansenshogaku Zasshi. 1996;70:4250.PubMedGoogle Scholar
  4. Yasin  RM, Jegathesan  MM, Tiew  CC. Salmonella serotypes isolated in Malaysia over the ten-year period 1983-1992. Asia Pac J Public Health. 1996-97;9:15.PubMedGoogle Scholar
  5. Rusul  G, Khair  J, Radu  S, Cheah  CT, Yassin  RM. Prevalence of Salmonella in broilers at retail outlets, processing plants and farms in Malaysia. Int J Food Microbiol. 1996;33:18394. DOIPubMedGoogle Scholar
  6. Sasipreeyajan  J, Jerngklinchan  J, Koowatananukul  C, Saitanu  K. Prevalence of salmonellae in broiler, layer and breeder flocks in Thailand. Trop Anim Health Prod. 1996;28:17480.PubMedGoogle Scholar
  7. Morse  LJ, Rubenstein  AD. A food-borne institutional outbreak of enteritis due to Salmonella blockley. JAMA. 1967;202:93940. DOIPubMedGoogle Scholar
  8. Albert  S, Weber  B, Schafer  V, Rosenthal  P, Simonsohn  M, Doerr  HW. Six enteropathogens from a case of acute gastroenteritis. Infection. 1990;18:3812. DOIPubMedGoogle Scholar
  9. van Duijkeren  E, Sloet van Oldruitenborgh-Oosterbaan  MM, Houwers  DJ, van Leeuwen  WJ, Kalsbeek  HC. Equine salmonellosis in a Dutch veterinary teaching hospital. Vet Rec. 1994;135:24850.PubMedGoogle Scholar
  10. Mallaret  MR, Turquand  O, Blatier  JF, Croize  J, Gledel  J, Micoud  M, Human salmonellosis and turtles in France. Rev Epidemiol Sante Publique. 1990;38:715.PubMedGoogle Scholar
  11. Levre  E, Valentini  P, Brunetti  M, Sacchelli  F. Stationary and migratory avifauna as reservoirs of Salmonella, Yersinia and Campylobacter. Ann Ig. 1989;1:72940.PubMedGoogle Scholar
  12. Morinigo  MA, Cornax  R, Castro  D, Jimenez-Notaro  M, Romero  P, Borrego  JJ. Antibiotic resistance of Salmonella strains isolated from natural polluted waters. J Appl Bacteriol. 1990;68:297302.PubMedGoogle Scholar
  13. Garcia-Villanova Ruiz  B, Cueto Espinar  A, Bolanos Carmona  MJ. A comparative study of strains of salmonella isolated from irrigation waters, vegetables and human infections. Epidemiol Infect. 1987;98:2716. DOIPubMedGoogle Scholar
  14. Matsushita  S, Yamada  S, Inaba  M, Kusunoki  J, Kudoh  Y, Ohashi  M. Serovar distribution and drug resistance of Salmonella isolated from imported and domestic cases in 1980-1989 in Tokyo. Kansenshogaku Zasshi. 1992;66:32739.PubMedGoogle Scholar
  15. Bangtrakulobth  A, Suthienkul  O, Kitjakara  A, Pornrungwong  S, Siripanichgon  K. First isolation of Salmonella blockley in Thailand. Southeast Asian J Trop Med Public Health. 1994;25:68892.PubMedGoogle Scholar
  16. Atanassova  V, Matthes  S, Muhlbauer  E, Helmuth  R, Schroeter  A, Ellendorff  F. Plasmid profiles of different Salmonella serovars from poultry flocks in Germany. Berl Munch Tierarztl Wochenschr. 1993;106:4047.PubMedGoogle Scholar
  17. Hamouda  O. Outbreak of Salmonella blockley infections in Germanypreliminary investigation results. Eurosurveillance Weekly 1998;2:980924. Available at URL:
  18. Benons  L. Salmonella blockley infections in England and Wales, 1998. Eurosurveillance Weekly 1998;2:980924. Available at URL:
  19. Vassilogiannakopoulos  A, Tassios  P, Lampiri  M. Salmonella blockley infection in Greece. Eurosurveillance Weekly 1999;3:990408. Available at URL:
  20. Kauffman  F. Serological diagnosis of Salmonella species. Copenhagen (Denmark): Munksgaard; 1972.
  21. National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard M7-A2. Wayne (PA): The Committee; 1997.
  22. Tassios  PT, Vatopoulos  AC, Mainas  E, Gennimata  D, Papadakis  J, Tsiftsoglou  A, Molecular analysis of ampicillin-resistant sporadic Salmonella typhi and Salmonella paratyphi B clinical isolates. Clin Microbiol Infect. 1997;3:31723. DOIPubMedGoogle Scholar
  23. Tenover  FC, Arbeit  RD, Goering  RV, Mickelsen  PA, Murray  BE, Persing  DH, Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995;33:22339.PubMedGoogle Scholar
  24. Tassios  PT, Markogiannakis  A, Vatopoulos  AC, Katsanikou  E, Velonakis  EN, Kourea-Kremastinou  K, Molecular epidemiology of antibiotic resistance of Salmonella enteritidis during a 7-year period in Greece. J Clin Microbiol. 1997;35:131621.PubMedGoogle Scholar

Main Article

Page created: May 09, 2011
Page updated: May 09, 2011
Page reviewed: May 09, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.