Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 10, Number 3—March 2004
Dispatch

Endemic Carbapenem-resistant Pseudomonas aeruginosa with Acquired Metallo-β-lactamase Determinants in European Hospital

Cristina Lagatolla*Comments to Author , Enrico A. Tonin*, Carlo Monti-Bragadin*, Lucilla Dolzani*, Francesca Gombac*, Claudia Bearzi*, Elisabetta Edalucci*, Fabrizia Gionechetti*, and Gian Maria Rossolini†
Author affiliations: *Università di Trieste, Trieste, Italy; †Università di Siena, Siena, Italy

Main Article

Figure

RAPD profiles of blaVIM positive strains. Amplification products (8 μL) obtained with primer 208 (5′-ACGGCCGACC-3′) (14) were run on 2% agarose gel. Lanes A-E: RAPD-types as indicated in Table 1. Lanes M: λDNA digested with EcoRI and HindIII.

Figure. RAPD profiles of blaVIM positive strains. Amplification products (8 μL) obtained with primer 208 (5′-ACGGCCGACC-3′) (14) were run on 2% agarose gel. Lanes A-E: RAPD-types as indicated in Table 1. Lanes M: λDNA digested with EcoRI and HindIII.

Main Article

References
  1. Livermore  DM, Woodford  N. Carbapenemases: a problem in waiting? Curr Opin Microbiol. 2000;3:489–95. DOIPubMedGoogle Scholar
  2. Bush  K. New β-lactamases in gram-negative bacteria: diversity and impact on the selection of antimicrobial therapy. Clin Infect Dis. 2001;32:1085–9. DOIPubMedGoogle Scholar
  3. Nordmann  P, Poirel  L. Emerging carbapenemases in Gram-negative aerobes. Clin Microbiol Infect. 2002;8:321–31. DOIPubMedGoogle Scholar
  4. Osano  E, Arakawa  Y, Wacharotayankun  R, Ohta  M, Horii  T, Ito  H, Molecular characterization of an enterobacterial metallo-β-lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance. Antimicrob Agents Chemother. 1994;38:71–8.PubMedGoogle Scholar
  5. Lauretti  L, Riccio  ML, Mazzariol  A, Cornaglia  G, Amicosante  G, Fontana  R, Cloning and characterization of blaVIM, a new integron-borne metallo-β-lactamase gene from a Pseudomonas aeruginosa clinical isolate. Antimicrob Agents Chemother. 1999;43:1584–90.PubMedGoogle Scholar
  6. Senda  K, Arakawa  Y, Nakashima  K, Ito  I, Ichiyama  S, Shimokata  K, Multifocal outbreaks of metallo-β-lactamase-producing Pseudomonas aeruginosa resistant to broad-spectrum β-lactams, including carbapenems. Antimicrob Agents Chemother. 1996;40:349–53.PubMedGoogle Scholar
  7. Senda  K, Arakawa  Y, Ichiyama  S, Nakashima  K, Ito  H, Ohsuka  S, PCR detection of metallo-β-lactamase gene (blaIMP) in gram-negative rods resistant to broad-spectrum β-lactams. J Clin Microbiol. 1996;34:2909–13.PubMedGoogle Scholar
  8. Cornaglia  G, Mazzariol  A, Lauretti  L, Rossolini  GM, Fontana  R. Hospital outbreak of carbapenem-resistant Pseudomonas aeruginosa producing VIM-1, a novel transferable metallo-β-lactamase. Clin Infect Dis. 2000;31:1119–25. DOIPubMedGoogle Scholar
  9. Hirakata  Y, Izumikawa  K, Yamaguchi  T, Takemura  H, Tanaka  H, Yoshida  R, Rapid detection and evaluation of clinical characteristics of emerging multiple-drug-resistant gram-negative rods carrying the metallo-β-lactamase gene blaIMP. Antimicrob Agents Chemother. 1998;42:2006–11.PubMedGoogle Scholar
  10. Lee  K, Lim  JB, Yum  JH, Yong  D, Chong  Y, Kim  JM, blaVIM-2 cassette-containing novel integrons in metallo-β-lactamase-producing Pseudomonas aeruginosa and Pseudomonas putida isolates disseminated in a Korean hospital. Antimicrob Agents Chemother. 2002;46:1053–8. DOIPubMedGoogle Scholar
  11. Tsakris  A, Pournaras  S, Woodford  N, Palepou  MF, Babini  GS, Duoboyas  J, Outbreak of infections caused by Pseudomonas aeruginosa producing VIM-1 carbapenemase in Greece. J Clin Microbiol. 2000;38:1290–2.PubMedGoogle Scholar
  12. Rossolini  GM, Riccio  ML, Cornaglia  G, Pagani  L, Lagatolla  C, Selan  L, Carbapenem-resistant Pseudomonas aeruginosa with acquired blaVIM metallo-β-lactamase determinants, Italy. Emerg Infect Dis. 2000;6:312–3. DOIPubMedGoogle Scholar
  13. Migliavacca  R, Docquier  JD, Mugnaioli  C, Amicosante  G, Daturi  R, Lee  K, Simple microdilution test for detection of metallo-beta-lactamase production in Pseudomonas aeruginosa. J Clin Microbiol. 2002;40:4388–90. DOIPubMedGoogle Scholar
  14. Mahenthiralingam  E, Campbell  ME, Foster  J, Lam  JS, Speert  DP. Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol. 1996;34:1129–35.PubMedGoogle Scholar
  15. Speijer  H, Savelkoul  PHM, Bonten  MJ, Stobberingh  EE, Tjhie  JH. Application of different genotyping methods for Pseudomonas aeruginosa in a setting of endemicity in an intensive care unit. J Clin Microbiol. 1999;37:3654–61.PubMedGoogle Scholar

Main Article

Page created: February 08, 2011
Page updated: February 08, 2011
Page reviewed: February 08, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external