Volume 16, Number 3—March 2010
Dispatch
Influenza A Pandemic (H1N1) 2009 Virus Infection in Domestic Cat
Table 1
Oligonucleotide sequences for primers and probes and dye labels used in novel molecular testing for pandemic (H1N1) 2009 virus, Iowa State University Veterinary Diagnostic Laboratory, Ames, Iowa, USA, 2009*
Name | Sequence (5′ → 3′) | Description |
---|---|---|
Influenza A NP screening assay | ||
SIVRTF | CGGACGAAAAGGCAACGA | NP forward primer |
SIVRTR | CTGCATTGTCTCCGAAGAAATAAG | NP reverse primer |
SIVRTP |
CCGATCGTGCCYTC |
NP probe, MGB FAM |
Pandemic influenza M differentiation assay | ||
M_F | TCAGGCCCCCTCAAAGC | M forward primer |
M_R1 | CATTCCATGAGAGCCTCAAGATC | M reverse primer 1 |
M_R1a | CACTCCATGAGAGCCTCAAGATC | M reverse primer 2 |
M_R1b | CATTCCATGAGTGCCTCAAGATC | M reverse primer 3 |
M_EUPr | CAGAGACTGGAAAGTGT | EU M MGB, VIC |
M_NAPr | CAGAGACTYGAAGAYGT | NA M MGB, FAM |
*Primers and MGB probes were obtained from Integrated DNA Technologies (Coralville, IA, USA) and Applied Biosystems Inc. (Foster City, CA, USA), respectively. SIV, swine influenza virus; NP, nucleoprotein; M, matrix.