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Volume 22, Number 11—November 2016
Research

Ambulatory Pediatric Surveillance of Hand, Foot and Mouth Disease as Signal of an Outbreak of Coxsackievirus A6 Infections, France, 2014–2015

Audrey MirandComments to Author , François Vié le Sage, Bruno Pereira, Robert Cohen, Corinne Levy, Christine Archimbaud, Hélène Peigue-Lafeuille, Jean-Luc Bailly, and Cécile Henquell
Author affiliations: Centre Hospitalier Universitaire de Clermont-Ferrand, Clermont-Ferrand, France (A. Mirand, B. Pereira, C. Archimbaud, H. Lafeuille, J.-L. Bailly, C. Henquell); Université d’Auvergne, Clermont-Ferrand (A. Mirand, C. Archimbaud, H. Peigue-Lafeuille, J.-L. Bailly, C. Henquell); Association Française de Pédiatrie Ambulatoire, Bagnols-sur-Cèze, France (F. Vié le Sage); Association Clinique et Thérapeutique Infantile du Val de Marne, Saint Maur des Fossés, France (R. Cohen, C. Levy); Université Paris Est, Créteil, France (R. Cohen, C. Levy)

Main Article

Figure 1

Methodologic approach for enterovirus genotyping and distribution of types associated with hand, foot and mouth disease and herpangina, France, April 2014–March 2015. A) Semi-nested reverse transcription PCR (RT-PCR) A using primers specifically developed for enterovirus types belonging to the EV-A species was first performed for all clinical samples except 1. For this sample, the viral load was low, and the nested RT-PCR described by Nix et al. (27) was performed directly. If the semi-nested RT

Figure 1. Methodologic approach for enterovirus genotyping and distribution of types associated with hand, foot and mouth disease and herpangina, France, April 2014–March 2015. A) Semi-nested reverse transcription PCR (RT-PCR) A using primers specifically developed for enterovirus types belonging to the EV-A species was first performed for all clinical samples except 1. For this sample, the viral load was low, and the nested RT-PCR described by Nix et al. (27) was performed directly. If the semi-nested RT-PCR A was negative, the genotyping was alternatively performed by a semi-nested RT-PCR B with primers specific to the EV-B species (28) or a nested RT-PCR (27). B) Among other EV-A species, 5 different types were identified: coxsackievirus (CV) A4, n = 18; CV-A8, n = 16; CV-A2 and CV-A5, n = 5 each; and CV-A12, n = 1. Among EV-B species, 12 different types were identified: echovirus (E) 16 (E-16) and E-18 (n = 5 each); E-11 and coxsackievirus B3 (CV-B3; n = 4 each); CV-B1, CV-B2, CV-B4, CV-A9, and E-6 (n = 2 each); and E-3, E-5, and E-25 (n = 1 each). EV-A, Enterovirus A; EV-A71, enterovirus A71; EV-B, Enterovirus B; RT-PCR, reverse transcription PCR.

Main Article

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Page created: October 18, 2016
Page updated: October 18, 2016
Page reviewed: October 18, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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