, Judith M.A. van den Brand1
, W. Widagdo1
, Marta Muñoz, Stalin Raj, Debby Schipper, David Solanes, Ivan Cordón, Albert Bensaid, Bart L. Haagmans, and Joaquim Segalés
Figure 3. Histology and expression of viral antigen (IHC) and viral RNA (ISH) at postinoculation day 4 in the nasal respiratory epithelium of sheep, pigs, llamas, and horses inoculated with MERS-CoV. A mild to severe rhinitis with epithelial necrosis and hypertrophy and inflammation of the epithelium and lamina propria was observed in the nasal respiratory tissue of pigs and llamas. Associated with these was presence of virus antigen (IHC) and RNA (ISH). No substantial lesions, virus antigen, or virus RNA were detected in the nasal respiratory tissues of sheep and horses (HE, IHC, ISH). Original magnification ×200 for all images. HE, hematoxylin and eosin; IHC, immunohistochemistry; ISH, in situ hybridization; MERS-CoV, Middle East respiratory syndrome coronavirus.