Volume 24, Number 6—June 2018
Reassortant Clade 22.214.171.124 of Highly Pathogenic Avian Influenza A(H5N6) Virus, Taiwan, 2017
A highly pathogenic avian influenza A(H5N6) virus of clade 126.96.36.199 was detected in a domestic duck found dead in Taiwan during February 2017. The endemic situation and continued evolution of various reassortant highly pathogenic avian influenza viruses in Taiwan warrant concern about further reassortment and a fifth wave of intercontinental spread.
Since 1996, H5 A/goose/Guangdong/1/1996 (Gs/GD) lineage of highly pathogenic avian influenza viruses (HPAIVs) originating in Asia have caused outbreaks in Asia, Europe, Africa, and North America (1). The H5N1 Gs/GD lineage of HPAIV has evolved into 10 genetically distinct virus clades (0–9) and multiple subclades, including novel H5 clade 188.8.131.52 viruses, which emerged in China (2) and have evolved into 4 distinct genetic groups (184.108.40.206A–D) (3). Four 4 intercontinental waves of Gs/GD lineage HPAIV transmission have occurred: clade 2.2 H5N1 in 2005, clade 220.127.116.11c H5N1 in 2009, clade 18.104.22.168A H5Nx in 2014, and clade 22.214.171.124B H5Nx in 2016 (4). The clade 126.96.36.199A and B H5N8 viruses spread intercontinentally; clade 188.8.131.52A caused outbreaks in Asia, Europe, and North America during 2014–2015, and clade 184.108.40.206B H5N8 caused outbreaks in Asia, Europe, and Africa during 2016–2017 (1,5). In fall 2016, clade 220.127.116.11C H5N6 viruses caused outbreaks in South Korea and Japan (6). Six distinct genotypes of clade 18.104.22.168C H5N6 viruses (designated as C1–C6) were identified in South Korea and Japan during these outbreaks; these genotypes contain different polymerase acidic and nonstructural genes from low pathogenicity influenza viruses from Eurasia (7,8).
We report HPAIV H5N6 detection from a meat-type duck in Taiwan in February 2017. One dead young Pekin-type domestic duck was found on a country road near the Xiuguluan River in Hualien County during wild bird and habitat surveillance for HPAIV by the Wild Bird Society of Taipei; the carcass was forwarded to the national laboratory of the Animal Health Research Institute (Technical Appendix 1 Figure 1). We conducted complete genome sequencing and comparative phylogenetic analysis of the detected virus, A/duck/Taiwan/1702004/2017(H5N6) (Dk/Tw/17), to trace the origin and understand its genetic features.
We detected Dk/Tw/17 virus by using reverse transcription PCR and isolated the virus by using egg inoculation as described previously (9). We conducted an intravenous pathogenicity index test according to the World Organisation for Animal Health Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (http://www.oie.int/en/international-standard-setting/terrestrial-manual). We performed full-length genome sequencing by using reverse transcription PCR amplification and Sanger sequencing (9). We estimated maximum-likelihood phylogenies by using RAxML (10) and constructed a median-joining phylogenetic network of the hemagglutinin gene by using NETWORK 5.0 (Technical Appendix 2).
We classified Dk/Tw/17 as an HPAIV on the basis of the amino acid sequence at the hemagglutinin cleavage site (PLRERRRKR/G) and its high lethality in chickens (intravenous pathogenicity index 3.0). Necropsy and histologic examination revealed virus-specific necrotic and inflammatory lesions in the pancreas, heart, and brain (Technical Appendix 1 Figure 2). Phylogenetic analyses suggested that the Dk/Tw/17 virus belongs to clade 22.214.171.124C genotype C5 that was found in China, South Korea, and Japan during 2016–2017 (Figure, panel A; Technical Appendix 1 Figure 1). This virus genotype acquired its polymerase acidic gene of low pathogenicity influenza viruses from Eurasia; its other genes originated in the G1.1.9 and G1.1-like lineages of H5N6 viruses from China (7,8). All 8 gene segments shared high levels of nucleotide identity (99.3%–99.9%) with H5N6 viruses identified from wild birds in Japan and South Korea in November 2016, including A/whooper swan/Korea/Gangjin 49-1/2016 (H5N6), A/spot billed duck/Korea/WB141/2016 (H5N6), and A/teal/Tottori/2/2016 (H5N6) (Technical Appendix 1 Table). These viruses consistently clustered together with high bootstrap value (>70) in maximum-likelihood phylogenies across all 8 gene segments (Technical Appendix 1 Figures 3–10).
The genotype C5 comprises 17 H5N6 HPAIVs identified from wild waterfowl in China, Japan, and South Korea during November–December 2016; a virus identified from a chicken farm (A/chicken/Korea/H23/2016 [H5N6]) in South Korea in November 2016; and the Dk/Tw/17 virus. Genotype C5 is phylogenetically distinct from viruses that caused outbreaks in poultry farms in Japan and South Korea during 2016–2017. This genotype has independently evolved and been maintained in wild bird populations in the bird flyway of East Asia, highlighting how wild waterfowl play an important role in the maintenance and dissemination of this HPAIV. In addition, the median-joining phylogenetic network analysis suggests that the A/chicken/Korea/H23/2016 (H5N6) is not the direct ancestor of the Dk/Tw/17 virus, which was likely caused by separate introduction from wild birds (Figure, panel B).
The site where the dead duck was collected is adjacent to a river and located near many ponds used for duck farming. After identification of Dk/Tw/17, intensified active surveillance conducted over 3 months detected additional clade 126.96.36.199C H5N6 HPAIVs from 12 farms in 4 counties (Technical Appendix 1 Figure 1). Clade 188.8.131.52A H5Nx HPAIVs, mainly H5N2 and H5N8, have caused outbreaks in the poultry industry of Taiwan since January 2015 (9). In 2017, clade 184.108.40.206A H5Nx and 220.127.116.11C H5N6 HPAIVs were detected in domestic poultry. The endemic situation and continued evolution of various reassortant HPAIVs in domestic poultry warrants concern about further reassortment. Enhanced active surveillance in domestic and wild waterfowl is required to monitor the spread and onward reassortment in Taiwan and to inform the design of improved prevention and control strategies.
Dr. Li-Hsuan Chen is an assistant research fellow at the Epidemiology Division, Animal Health Research Institute, New Taipei City, Taiwan. Her main research interests include diagnosis of poultry diseases and the phylogenetic and phenotypic characterization of avian influenza viruses.
Dr. Dong-Hun Lee is a postdoctoral researcher at the Southeast Poultry Research Laboratory, USDA Agricultural Research Service, Athens, Georgia, USA. His research interests include molecular epidemiology and host–pathogen interaction of avian viruses.
We thank the Wild Bird Society of Taipei for submitting cases and contributing to the early detection of disease. Acknowledgments for laboratory contributions by GISAID partners are listed in online Technical Appendix 2 (https://wwwnc.cdc.gov/EID/article/24/6/17-2071-Techapp2.xlsx).
This work was funded by the Council of Agriculture of Taiwan.
- Lee DH, Bertran K, Kwon JH, Swayne DE. Evolution, global spread, and pathogenicity of highly pathogenic avian influenza H5Nx clade 18.104.22.168. J Vet Sci. 2017;18(S1):269–80.
- Zhao G, Gu X, Lu X, Pan J, Duan Z, Zhao K, et al. Novel reassortant highly pathogenic H5N2 avian influenza viruses in poultry in China. PLoS One. 2012;7:e46183.
- Lee DH, Bahl J, Torchetti MK, Killian ML, Ip HS, DeLiberto TJ, et al. Highly pathogenic avian influenza viruses and generation of novel reassortants, United States, 2014–2015. Emerg Infect Dis. 2016;22:1283–5.
- Sims L, Harder T, Brown I, Gaidet N, Belot G. Dobschuetz Sv, et al. Highly pathogenic H5 avian influenza in 2016 and 2017—observations and future perspectives [cited 2017 Dec 15]. http://agritrop.cirad.fr/585953
- Global Consortium for H5N8 and Related Influenza Viruses. Role for migratory wild birds in the global spread of avian influenza H5N8. Science. 2016;354:213–7.
- Okamatsu M, Ozawa M, Soda K, Takakuwa H, Haga A, Hiono T, et al. Characterization of highly pathogenic avian influenza virus A(H5N6), Japan, November 2016. Emerg Infect Dis. 2017;23:691–5.
- Takemae N, Tsunekuni R, Sharshov K, Tanikawa T, Uchida Y, Ito H, et al. Five distinct reassortants of H5N6 highly pathogenic avian influenza A viruses affected Japan during the winter of 2016-2017. Virology. 2017;512:8–20.
- Lee EK, Song BM, Lee YN, Heo GB, Bae YC, Joh SJ, et al. Multiple novel H5N6 highly pathogenic avian influenza viruses, South Korea, 2016. Infect Genet Evol. 2017;51:21–3.
- Lee MS, Chen LH, Chen YP, Liu YP, Li WC, Lin YL, et al. Highly pathogenic avian influenza viruses H5N2, H5N3, and H5N8 in Taiwan in 2015. Vet Microbiol. 2016;187:50–7.
- Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics. 2014;30:1312–3.
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Original Publication Date: May 04, 2018
1These authors contributed equally to this article.