Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 25, Number 10—October 2019
Research Letter

Lassa Virus in Pygmy Mice, Benin, 2016–2017

Anges Yadouleton, Achaz Agolinou, Fodé Kourouma, Raoul Saizonou, Meike Pahlmann, Sonia Kossou Bedié, Honoré Bankolé, Beate Becker-Ziaja, Fernand Gbaguidi, Anke Thielebein, N’Faly Magassouba, Sophie Duraffour, Jean-Pierre Baptiste, Stephan Günther, and Elisabeth Fichet-CalvetComments to Author 
Author affiliations: Laboratoire des Fièvres Hémorragiques Virales, Cotonou, Benin (A. Yadouleton, A. Agolinou); Ministry of Health, Cotonou (A. Yadouleton, H. Bankolé, F. Gbaguidi); Laboratoire des Fièvres Hémorragiques Virales, Conakry, Guinea (F. Kourouma, N. Magassouba); World Health Organization, Cotonou (R. Saizonou, S.K. Bedié, J.-P. Baptiste); Bernhard-Nocht Institute for Tropical Medicine, Hamburg, Germany (M. Pahlmann, B. Becker-Ziaja, A. Thielebein, S. Duraffour, S. Günther, E. Fichet-Calvet)

Main Article

Figure

Bayesian phylogenetic analyses based on nucleotide sequences of the partial glycoprotein and nucleoprotein genes of Lassa virus (LASV), showing the placement of the new sequences (in boldface) isolated from Mus baoulei pygmy mice, in comparison with other sequences representing the members of LASV lineages I–IV. A) Glycoprotein, 1,408 nt; B) nucleoprotein, 1,654 nt. The trees are rooted by Gbagroube, a LASV-like virus isolated from Mus setulosus mice in Côte d’Ivoire. Statistical support of grou

Figure. Bayesian phylogenetic analyses based on nucleotide sequences of the partial glycoprotein and nucleoprotein genes of Lassa virus (LASV), showing the placement of the new sequences (in boldface) isolated from Mus baoulei pygmy mice, in comparison with other sequences representing the members of LASV lineages I–IV. A) Glycoprotein, 1,408 nt; B) nucleoprotein, 1,654 nt. The trees are rooted by Gbagroube, a LASV-like virus isolated from Mus setulosus mice in Côte d’Ivoire. Statistical support of grouping from Bayesian posterior probabilities is indicated at the nodes. Country, strain names, and GenBank accession numbers are indicated on the branches. The analysis was inferred by using the Bayesian Markov chain Monte Carlo method implemented in BEAST (8). The following settings were used: general time reversible plus gamma, strict clock, and constant population. Markov chain Monte Carlo chains were run for 10 million states and sampled every 10,000 states to obtain an effective sample size >200 for all parameters. The new viral and murine sequences are deposited under accession nos. MH028396–404. Scale bars indicate nucleotide substitutions per site.

Main Article

References
  1. Whitmer  SLM, Strecker  T, Cadar  D, Dienes  HP, Faber  K, Patel  K, et al. New lineage of Lassa virus, Togo, 2016. Emerg Infect Dis. 2018;24:599602. DOIPubMedGoogle Scholar
  2. World Health Organization. Lassa fever–Benin [cited 2016 July 1]. http://wwwwhoint/csr/don/13-june-2016-lassa-fever-benin
  3. Fichet-Calvet  E. Lassa fever: a rodent-human interaction. In: Johnson N, editor. The role of animals in emerging viral diseases. London: Elsevier; 2014. p. 89–123.
  4. Kronmann  KC, Nimo-Paintsil  S, Guirguis  F, Kronmann  LC, Bonney  K, Obiri-Danso  K, et al. Two novel arenaviruses detected in pygmy mice, Ghana. Emerg Infect Dis. 2013;19:18325. DOIPubMedGoogle Scholar
  5. Olayemi  A, Obadare  A, Oyeyiola  A, Fasogbon  A, Igbokwe  J, Igbahenah  F, et al. Small mammal diversity and dynamics within Nigeria, with emphasis on reservoirs of the Lassa virus. Syst Biodivers. 2017;15:110.
  6. Olschläger  S, Lelke  M, Emmerich  P, Panning  M, Drosten  C, Hass  M, et al. Improved detection of Lassa virus by reverse transcription-PCR targeting the 5′ region of S RNA. J Clin Microbiol. 2010;48:200913. DOIPubMedGoogle Scholar
  7. Vieth  S, Drosten  C, Lenz  O, Vincent  M, Omilabu  S, Hass  M, et al. RT-PCR assay for detection of Lassa virus and related Old World arenaviruses targeting the L gene. Trans R Soc Trop Med Hyg. 2007;101:125364. DOIPubMedGoogle Scholar
  8. Drummond  AJ, Suchard  MA, Xie  D, Rambaut  A. Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol. 2012;29:196973. DOIPubMedGoogle Scholar
  9. Bowen  MD, Rollin  PE, Ksiazek  TG, Hustad  HL, Bausch  DG, Demby  AH, et al. Genetic diversity among Lassa virus strains. J Virol. 2000;74:69927004. DOIPubMedGoogle Scholar
  10. Olayemi  A, Cadar  D, Magassouba  N, Obadare  A, Kourouma  F, Oyeyiola  A, et al. New hosts of the Lassa virus. Sci Rep. 2016;6:25280. DOIPubMedGoogle Scholar

Main Article

Page created: September 17, 2019
Page updated: September 17, 2019
Page reviewed: September 17, 2019
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external