Early Diagnosis of Tularemia by Flow Cytometry, Czech Republic, 2003–20151
, Pavlína Tinavská, Olga Dvořáčková, Pavlína Filipová, Věra Hnetilová, Pavel Žampach, Květoslava Batistová, Václav Chmelík, Amanda E. Semper, and Nick J. Beeching
Author affiliations: České Budějovice Hospital, České Budějovice, Czech Republic (A. Chrdle, P. Tinavská, P. Filipová, V. Hnetilová, P. Žampach, V. Chmelík); University of South Bohemia Faculty of Health and Social Sciences, České Budějovice, Czech Republic (A. Chrdle, O. Dvořáčková); Royal Liverpool University Hospital, Liverpool, UK (A. Chrdle, N.J. Beeching); Písek Hospital, Písek, Czech Republic (K. Batistová); Public Health England, Porton Down, UK (A.E. Semper); National Institute for Health Research Health Protection Research Unit in Emerging and Zoonotic Infections, Liverpool, UK (A.E. Semper; N.J. Beeching); Liverpool School of Tropical Medicine, Liverpool (N.J. Beeching)
Figure 4. Percentage of CD3+ lymphocytes that are γδ T cells and CD3+/CD4–/CD8– T cells in peripheral blood samples from patients with confirmed or probable tularemia by clinical manifestation, Czech Republic, 2003–2015. The percentage of γδ T cells was determined for 48 cases and percentage of CD3+/CD4–/CD8– T cells for 64 cases. Paired comparisons (Kruskal-Wallis test) reveal no significant differences except for glandular versus typhoidal in γδ (p = 0.037) and CD3+/CD4–/CD8– T cells (p = 0.041). Boxes indicate interquartile ranges (IQRs), horizontal lines within boxes indicate medians, whiskers indicate range values <1.5× the IQR limits, and circles indicate outliers (i.e., values >1.5× the IQR limits).
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