Human Outbreak of Trichinellosis Caused by Trichinella papuae Nematodes, Central Kampong Thom Province, Cambodia
, Sotharith Bory, Michel Pluot, Mary Nheb, Sarin Chan, Sang Houn Prum, Sun Bun Hong Lim, Mala Sim, Yi Sengdoeurn, Ly Sovann, Virak Khieu, Isabelle Vallée, and Hélène Yera
Author affiliations: Institut Pasteur, Phnom Penh, Cambodia (Y. Caron, M. Sim); Calmette Hospital, Phnom Penh (S. Bory, M. Pluot, M. Nheb, S. Chan); Preah Ket Mealea Hospital, Phnom Penh (S. H. Prum); Kampong Thom Province Hospital, Kampong Thom, Cambodia (S.B.H. Lim); Ministry of Health, Phnom Penh (Y. Sengdoeun, L. Sovann, V. Khieu); Agence Nationale Sécurité Sanitaire Alimentaire Nationale, Maisons-Alfort, France (I. Vallée); Paris University, Paris, France (H. Yera)
Figure 1. Trichinella larvae samples from patients in Cambodia. A) Transverse section of a muscle (bicep) biopsy specimen from patient 13 showing a nonencapsulated T. papuae stage 1 nematode larva in the center of the specimen (hematoxylin phloxine saffron stain; scale bar = 200 μm). B) Higher magnification view of the same biopsy specimen showing the coelomyarian muscle structure and stichosome of Trichinella larvae (scale bar = 50 μm). C) T. papuae stage 1 nematode larva after deparaffinization and artificial digestion of muscle biopsy specimen (scale bar = 50 μm).
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