Pathology and Pathogenesis of SARS-CoV-2 Associated with Fatal Coronavirus Disease, United States
Roosecelis B. Martines1
, Jana M. Ritter1
, Eduard Matkovic, Joy Gary, Brigid C. Bollweg, Hannah Bullock, Cynthia S. Goldsmith, Luciana Silva-Flannery, Josilene N. Seixas, Sarah Reagan-Steiner, Timothy Uyeki, Amy Denison, Julu Bhatnagar, Wun-Ju Shieh, Sherif R. Zaki, and COVID-19 Pathology Working Group
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (R.B. Martines, J.M. Ritter, E. Matkovic, J. Gary, B.C. Bollweg, C.S. Goldsmith, L. Silva-Flannery, J.N. Seixas, S. Reagan-Steiner, T. Uyeki, A. Denison, J. Bhatnagar, W.-J. Shieh, S.R. Zaki); Synergy America Inc., Atlanta (H. Bullock).
Figure 5. Ultrastructural features of severe acute respiratory syndrome coronavirus 2 infection within the upper airway of a fatal coronavirus disease case from formalin-fixed paraffin-embedded (FFPE) tissue. Viral particles associated with the cilia of ciliated cells (A, C, and D) and the cytoplasm of respiratory epithelial cells (B) in the upper airway are indicted by arrows. Images in panels A and C were obtained from FFPE tissue removed from a paraffin block using a 2-mm biopsy punch. Images in panels B and D were collected from a 3 μm section of FFPE tissue affixed to a glass slide. Viral particles visualized in FFPE samples were smaller than those observed from fresh tissue; extracellular viral particles in fresh tissue samples were 105 nm in diameter and those from FFPE tissues were 75 nm in diameter. Scale bars indicate 1 μm (panel A), 800 nm (panel B), and 200 nm (panels C and D).
Page created: May 21, 2020
Page updated: August 18, 2020
Page reviewed: August 18, 2020
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