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Volume 29, Number 6—June 2023
Dispatch

MERS-CoV‒Specific T-Cell Responses in Camels after Single MVA-MERS-S Vaccination

Christian Meyer zu Natrup, Lisa-Marie Schünemann, Giulietta Saletti, Sabrina Clever, Vanessa Herder, Sunitha Joseph, Marina Rodriguez, Ulrich Wernery, Gerd Sutter, and Asisa VolzComments to Author 
Author affiliations: University of Veterinary Medicine Hannover, Hanover, Germany (C. Meyer zu Natrup, L.-M. Schünemann, G. Saletti, S. Clever, A. Volz); University of Glasgow, Glasgow, Scotland, UK (V. Herder); Central Veterinary Research Laboratory, Dubai, United Arab Emirates (S. Joseph, M. Rodriguez, U. Wernery); Ludwig Maximilian University Munich, Munich, Germany (G. Sutter)

Main Article

Figure 2

Antigen-specific cellular immunity after MVA-MERS-S vaccination in dromedary camels, Dubai, United Arab Emirates. PBMCs were isolated from blood samples on different days post‒single-shot vaccination and IFN-γ SFCs were measured by ELISpot assay after restimulation of PBMCs from different time points with overlapping peptides comprising the MERS-CoV S1 (A) and MERS-CoV-S2 (B) protein subunit. Box plots show individual values (dots), median values (horizontal lines within boxes), first and third quartiles (box tops and bottoms), and minimums and maximums of value distribution (error bars). IFN-γ, interferon-γ; MERS-CoV, Middle East respiratory syndrome coronavirus; MVA, modified vaccinia virus Ankara; MVA-MERS-S, modified vaccinia virus Ankara expressing full-length MERS-CoV spike protein as antigen; PBMCs, peripheral blood mononuclear cells; SFCs, spot-forming T cells.

Figure 2. Antigen-specific cellular immunity after MVA-MERS-S vaccination in dromedary camels, Dubai, United Arab Emirates. PBMCs were isolated from blood samples on different days post‒single-shot vaccination and IFN-γ SFCs were measured by ELISpot assay after restimulation of PBMCs from different time points with overlapping peptides comprising the MERS-CoV S1 (A) and MERS-CoV-S2 (B) protein subunit. Box plots show individual values (dots), median values (horizontal lines within boxes), first and third quartiles (box tops and bottoms), and minimums and maximums of value distribution (error bars). IFN-γ, interferon-γ; MERS-CoV, Middle East respiratory syndrome coronavirus; MVA, modified vaccinia virus Ankara; MVA-MERS-S, modified vaccinia virus Ankara expressing full-length MERS-CoV spike protein as antigen; PBMCs, peripheral blood mononuclear cells; SFCs, spot-forming T cells.

Main Article

Page created: March 29, 2023
Page updated: May 17, 2023
Page reviewed: May 17, 2023
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