Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 29, Number 8—August 2023
Dispatch

Candidatus Neoehrlichia mikurensis Infection in Patient with Antecedent Hematologic Neoplasm, Spain1

Paola González-Carmona23, Aránzazu Portillo2, Cristina Cervera-Acedo, Daniel González-Fernández, and José A. OteoComments to Author 
Author affiliations: Hospital de Jarrio, Asturias, Spain (P. González-Carmona, D. González-Fernández); Hospital Universitario San Pedro-CIBIR, La Rioja, Spain (A. Portillo, C. Cervera-Acedo, J.A. Oteo)

Main Article

Figure

Phylogenetic analysis of groEL gene from Candidatus Neoehrlichia mikurensis infecting a patient with antecedent hematologic neoplasm, Spain. Phylogenetic tree was generated to compare 809 bp fragments of the 60-kDa heat shock protein gene groEL from Candidatus Neoehrlichia mikurensis by using IQ-tree software version 2.2.0 (http://www.iqtree.org), maximum-likelihood method, and substitution model consisting of 3-parameter model 2 plus empirical base frequencies with rate heterogeneity allowing for a proportion of invariable sites. Values are approximate likelihood ratio test/bootstrap percentages, indicating topologic branch support for maximum-likelihood analysis with 1,000 replicates; values >75% define high stability. Diamond indicates nucleotide sequence of Candidatus N. mikurensis groEL gene fragment obtained in this study. Ehrlichia ruminantium (Anaplasmataceae family) groEL sequence was used as the outgroup. GenBank accession numbers are in parentheses. CNM, Candidatus N. mikurensis; I. ricinus, Ixodes ricinus; M. agrestis, Microtus agrestis. Scale bar indicates nucleotide substitutions per site.

Figure. Phylogenetic analysis of groEL gene from Candidatus Neoehrlichia mikurensis infecting a patient with antecedent hematologic neoplasm, Spain. Phylogenetic tree was generated to compare 809 bp fragments of the 60-kDa heat shock protein gene groEL from Candidatus Neoehrlichia mikurensis by using IQ-tree software version 2.2.0 (http://www.iqtree.org), maximum-likelihood method, and substitution model consisting of 3-parameter model 2 plus empirical base frequencies with rate heterogeneity allowing for a proportion of invariable sites. Values are approximate likelihood ratio test/bootstrap percentages, indicating topologic branch support for maximum-likelihood analysis with 1,000 replicates; values >75% define high stability. Diamond indicates nucleotide sequence of Candidatus N. mikurensis groEL gene fragment obtained in this study. Ehrlichia ruminantium (Anaplasmataceae family) groEL sequence was used as the outgroup. GenBank accession numbers are in parentheses. CNM, Candidatus N. mikurensis; I. ricinus, Ixodes ricinus; M. agrestis, Microtus agrestis. Scale bar indicates nucleotide substitutions per site.

Main Article

1Data from this study were presented at the joint LXIV National Conference of the Spanish Society of Hematology and Hemotherapy, XXXVIII National Conference of the Spanish Society of Thrombosis and Hemostasis, and 38th World Congress of the International Society of Hematology; October 6–8, 2022; Barcelona, Spain; and International Intracellular Bacteria Meeting; August 23–26, 2022; Lausanne, Switzerland.

2These first authors contributed equally to this article.

3Current affiliation: Hospital Universitario San Agustín, Asturias, Spain.

Page created: June 13, 2023
Page updated: July 20, 2023
Page reviewed: July 20, 2023
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external