Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 7, Number 6—December 2001
Research

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen*†Comments to Author , Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*
Author affiliations: *National Public Health Institute, Department in Turku, Finland;; †Turku University Central Hospital, Turku, Finland

Main Article

Table 1

Primers and probes used in study of Bordetella pertussis pertactin gene variants

Primer/probe Sequence (5'-3')a Positionb
QJF3c GCT GGT GCA GAC GCC AGT 1578-1595
QJR1c CCG ATA TCG ACC TTG CC 1649-1633
QH8Fd CTG CAG CGC GCG ACG ATA 757-774
QH2Rd ATT GCC GTG CGG TGC GGA CAA 1026-1006
QJ1e CCG GCG GTG CGG TTC C-F 809-824
QJ2e LC Red 640-CGG TGG TGC GGT TCC C-P 825-840

aModifications of primer or probe are boldfaced or underlined.
bPosition numbers indicate the position of bases relative to the first start codon of prn1.
cPrimers used in the real-time allele-specific amplification. QJF3 contained a specific mismatch G (underlined) at the 3'end that does not complement the published sequences of any prn type. The T (boldfaced) at the 3'end (corresponding to the nucleotide 1595) is complementary to prn1-5. Primer QJF3 has two mismatches with prn6-8.
dPrimers used in fluorescence resonance energy transfer (FRET) probe assay.
eProbes used in FRET probe assay. Boldfaced T is complementary to C to T transition specific for prn1. QJ1 was labeled with fluorescein at the 3' end and QJ2 with LC Red at 5'end and phosphorylated at the 3'end.

Main Article

Page created: December 09, 2010
Page updated: December 09, 2010
Page reviewed: December 09, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external