Volume 8, Number 1—January 2002
Prevalence and Genetic Profiling of Virulence Determinants of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Beef, and Humans, Calcutta, India
|Nucleotide sequence of primers||Target||PCR conditionsa
|5-GAGAATTCACAGATGGATATTTCAAATTTC-3 5-TCTCCTCGAGCTAGTTGACCTCGTTCAGAAACG-3||espP||94°C, 10s||68°C, 30s||72°C, 90s||2,900|
|5-GAGAGAATTCTTCCTGTTCTGATTCTTCTG-3 5-TCTCCTCGAGTCAAAACTTATTTCTCGCATCATCATCC-3||katP||94°C, 10s||68°C, 30s||72°C, 60s||2,130|
|5-TCTCAAGCTTTTAGAAATAGTCTCGCCAGTATTC-3 5-GAGAGAATTCGTCAGGAGGATGTTCAGG-3||eae||94°C, 10s||68°C, 30s||72°C, 90s||880|
|5-GAGAGGATCCGGTGCAGCAGAAAAAGTTGTA-3 5-TCTCCTCGAGTCATCTCGCCTGATAGTGTTTGG-3||hlyA||94°C, 10s||68°C, 30s||72°C, 60s||1,560|
aPCR was done for 30 cycles.
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