Figure 1. Touch impression of a rabies-positive Tanzanian domestic dog brain preserved in 50% glycerol saline solution for 15 months before testing by direct rapid immunohistochemical test (dRIT) and retested by direct fluorescent-antibody assay (DFA) after 5 months. A) Brain stained by dRIT: rabies virus antigen appears as magenta inclusions (arrowheads) against the blue neuronal hematoxylin counterstain. Magnification, ×630. B) Immunofluorescent apple-green viral inclusions in the same brain processed by DFA. Magnification, ×200.