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Volume 24, Number 1—January 2018

Emergence of Vaccine-Derived Polioviruses during Ebola Virus Disease Outbreak, Guinea, 2014–2015

Maria Dolores Fernandez-GarciaComments to Author , Manasi Majumdar, Ousmane Kebe, Aichatou D. Fall, Moussa Kone, Mouctar Kande, Moustapha Dabo, Mohamed Salif Sylla, Djenou Sompare, Wayne Howard, Ousmane Faye, Javier Martin1, and Kader Ndiaye1
Author affiliations: Institut Pasteur, Dakar, Senegal (M.D. Fernandez-Garcia, O. Kebe, A.D. Fall, O. Faye, K. Ndiaye); National Institute for Biological Standards and Control, Potters Bar, UK (M. Majumdar, J. Martin); World Health Organization, Conakry, Guinea (M. Kone, M. Kande); Guinean Ministry of Health, Conakry (M. Dabo, M.S. Sylla, D. Sompare); National Institute for Communicable Diseases, Johannesburg, South Africa (W. Howard)

Main Article

Table 5

Nucleotide and amino acid substitutions in the vaccine part of the genome of vaccine-derived polioviruses, Guinea, 2014–2015*

Genomic region, nt or aa position Sabin-2 15-114-C6 14-120 15-078 15-114 15-115 15-136 15-170 15-115-C4 15-115-C5 15-115-C8 15-115-C10 15-136-C1 15-136-C2 Phenotype
187 A G
202 C T
216 T C
290 G A
398 T C
438 A G
481 A G Attenuation
653 G C
654 G A
655 T C
696 T C
710 A G
713 C T
718 A G
725 G A


73 Ser Asn NAg site 3
75 Thr Ala NAg site 3

NAg site 3
4 Asp Asn Ser Gly Gly Gly
10 Val Ile Ile Ile Ile Ile Ile Ile Ile Ile Ile Ile Ile Ile
19 Val Ala Ala Ala Ala Ala
23 Ser Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro
24 Thr Ala
26 Ser Gly
30 Thr Ile Ile Ile Ile Ile
31 Lys Arg Arg Arg Arg Arg Arg Arg
43 Ala Val
103 Arg Lys Lys NAg site 1
143 Ile Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Attenuation
171 Asn Asp Asp Asp NAg site 1

*In the 5′-UTR, all nucleotide positions differentiating VDPV 15-114-C6 from Sabin-2 are shown. In the capsid proteins region (VP4–VP1), only amino acid changes are shown. In the VP4-VP3 capsid protein regions, all amino acid positions differentiating VDPV 15-114-C6 from Sabin-2 are shown. For the VP1 capsid protein region, all amino acid positions differentiating all VDPVs from Guinea isolated in this study are shown. NAg, neutralizing antigen; UTR, untranslated region; VDPV, vaccine-derived poliovirus; VP, viral capsid protein. Boldface indicates relevant nucleotide or amino acid sites in the Sabin-2 genome described as being involved in Sabin-2 attenuation (16) or those predicted NAg sites important for immune recognition (18,19); italics indicate amino acid positions; – indicates no amino acid change; blank cells indicate no sequencing data.

Main Article

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1These authors contributed equally to this article.

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