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Volume 25, Number 4—April 2019
Research

Sand Fly–Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, Kenya

David P. TchouassiComments to Author , Marco Marklewitz, Edith Chepkorir, Florian Zirkel1, Sheila B. Agha, Caroline C. Tigoi, Edith Koskei, Christian Drosten, Christian Borgemeister, Baldwyn Torto, Sandra Junglen2Comments to Author , and Rosemary Sang2
Author affiliations: International Centre of Insect Physiology and Ecology, Nairobi, Kenya (D.P. Tchouassi, E. Chepkorir, S.B. Agha, C.C. Tigoi, B. Torto, R. Sang); Charité-Universitätsmedizin Berlin, Berlin, Germany (M. Marklewitz, F. Zirkel, C. Drosten, S. Junglen); German Center for Infection Research, Berlin (M. Marklewitz, F. Zirkel, C. Drosten, S. Junglen); Center for Virus Research, Kenya Medical Research Institute, Nairobi (E. Koskei, R. Sang); University of Bonn, Bonn, Germany (C. Borgemeister)

Main Article

Figure 5

In vitro growth kinetics of novel sand fly–associated phlebovirus Ntepes virus from Kenya in different cell lines. A) Insects: LL-5, sand fly; C6/36, mosquito. B) Human: HEK293-T. C) Peridomestic wildlife: hamster, BHK-21; primate, VeroE6/7; mouse, MEF; bat, EidNi. D) Livestock: swine, PK-15; goat, ZN-R; chicken, DF-1; cattle, KN-R. Cells were infected with a multiplicity of infection of 0.1; supernatants were collected every 24 h for 7 d postinfection. Viral genome copies were measured at indic

Figure 5. In vitro growth kinetics of novel sand fly–associated phlebovirus Ntepes virus from Kenya in different cell lines. A) Insects: LL-5, sand fly; C6/36, mosquito. B) Human: HEK293-T. C) Peridomestic wildlife: hamster, BHK-21; primate, VeroE6/7; mouse, MEF; bat, EidNi. D) Livestock: swine, PK-15; goat, ZN-R; chicken, DF-1; cattle, KN-R. Cells were infected with a multiplicity of infection of 0.1; supernatants were collected every 24 h for 7 d postinfection. Viral genome copies were measured at indicated timepoints by real-time reverse transcription PCR. E) Pathogenicity of Ntepes virus infection in mice. Litters of 2-day-old Swiss Albino suckling mice (8 mice/litter) were intracerebrally inoculated using the indicated virus titers or cell culture media as a control. Animals were monitored daily for signs of disease. Titers are shown in PFU/mL.

Main Article

1Current affiliation: Biotest AG, Dreieich, Germany.

2These authors contributed equally to this article.

Page created: March 17, 2019
Page updated: March 17, 2019
Page reviewed: March 17, 2019
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