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Volume 25, Number 4—April 2019
Research

Differences in Neuropathogenesis of Encephalitic California Serogroup Viruses

Alyssa B. Evans, Clayton W. Winkler, and Karin E. PetersonComments to Author 
Author affiliations: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA

Main Article

Table 1

Cell death and replication kinetics of 5 encephalitic California serogroup viruses in SH-SY5Y cells*

Virus
 MOI 0.1
MOI 0.01
Difference in cell death†
Virus replication
 Difference in cell death†
Virus replication
Range, hpi
 p value range
 Replication rate p value‡
 Peak titer, PFU/mL
 hpi§
 Range, hpi
 p value range
 Replication rate p value‡
 Peak titer, PFU/mL
 hpi§
LACV Ref Ref Ref 1.93 × 108 48 Ref Ref Ref 3.51 × 108 96
SSHV NS NS 0.041 4.36 × 109 72 NS NS 0.038 4.82 × 109 72
TAHV NS NS 0.110 9.13 × 107 48 NS NS 0.995 1.05 × 108 48
JCV 61–96 0.047–0.0001 0.033 1.33 × 108 72 67–96 0.022–0.0001 0.034 1.09 × 108 96
INKV 64–96 0.024–0.0001 0.002 8.72 × 107 72 67–96 0.023–0.0001 0.023 1.55 × 108 96

*All statistical comparisons were made with LACV used as the reference. hpi, hour postinoculation; INKV, Inkoo virus; JCV, Jamestown Canyon virus; LACV, La Crosse virus; MOI, multiplicity of infection; NS, not significant; Ref, referent; SSHV, snowshoe hare virus; TAHV, Tahyna virus.
†Cell death for >3 independent experiments analyzed by 2-way analysis of variance.
‡Replication rates from 3 independent experiments were analyzed via linear regression analysis of slopes during the log phase of viral growth (6–48 hpi). Significant p values (<0.05) are in boldface.
§Time point at which the sampled peak titer was observed.

Main Article

Page created: March 18, 2019
Page updated: March 18, 2019
Page reviewed: March 18, 2019
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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