Volume 26, Number 4—April 2020
Research
Genomic Insight into the Spread of Meropenem-Resistant Streptococcus pneumoniae Spain23F-ST81, Taiwan
Table 2
Pneumococcal transformation study results for study of invasive Streptococcus pneumoniae isolates from 2 tertiary hospitals in Taiwan, 2013–2017*
Recipient | Transforming DNA | Transformant name | Colony formation | MIC of transformants, mg/L |
Integration of altered PBP in transformants | PBP profile 1A:2B:2X | |
---|---|---|---|---|---|---|---|
Meropenem | Penicillin | ||||||
R6 | NA | R6-WT | ND | 0.015 | 0.015 | ND | 2:0:2 |
15A-ST63 | NA | 15A-WT | ND | 1 | 4 | ND | 13:new1:43 |
R6 | pbp1a | R6–1A | − | ND | ND | ND | ND |
R6 | pbp2b | R6–2B | + | 0.06 | 0.015 | Yes, PBP2b | 2:new1:2 |
R6 | pbp2x | R6–2X | − | ND | ND | ND | ND |
R6–2B transformants | pbp2x | R6–2B-2X | + | 0.25 | 0.125 | Yes, PBP2x | 2:new1:43 |
R6–2B transformants | pbp1a | R6–2B-1A | − | ND | ND | ND | ND |
R6–2B-2X transformants | pbp1a | R6–2B-2X-1A | + | 0.5 | 1 | Yes, PBP1a | 13:new1:43 |
*NA, not applicable; ND, not done; PBP, penicillin binding protein; +, positive for colony formation on antimicrobial-containing agar plate; −, negative for colony formation on antimicrobial-containing agar plate.
1These first authors contributed equally to this article.