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Volume 27, Number 9—September 2021
Research

Development and Clinical Evaluation of a CRISPR-Based Diagnostic for Rapid Group B Streptococcus Screening

Lingxiao Jiang1, Weiqi Zeng1, Wanting Wu1, Yingying Deng, Fusheng He, Wenli Liang, Mingyao Huang, Hong Huang, Yongjun Li, Xiaorui Wang, Hang Su, Shilei PanComments to Author , and Teng Xu1Comments to Author 
Author affiliations: Zhujiang Hospital, Southern Medical University, Guangzhou, China (L. Jiang, Y. Deng, W. Liang, M. Huang); Vision Medicals Center for Medical Research, Shenzhen, China (W. Zeng, W. Wu, H. Huang, Y. Li, X. Wang, H. Su, T. Xu); Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province and College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China (W. Zeng, T. Xu); Zhujiang Hospital, Southern Medical University, Guangzhou (S. Pan)

Main Article

Figure 1

Schematic diagram of CRISPR-based diagnostic for rapid GBS screening. Swab samples are first eluted and followed by a rapid DNA extraction step where the bacterial cell walls are disrupted by a combination of chemical, physical, and heating effects. The extracted DNA is then subjected to the CRISPR/Cas reaction. The collateral nuclease activity of Cas proteins are activated upon specific binding of gRNA to the atoB gene. Fluorescent signal produced from cleaved probes is captured and indicates the presence of GBS. GBS, group B Streptococcus. gRNA, guide RNA; ssRNA, single-stranded RNA.

Figure 1. Schematic diagram of CRISPR-based diagnostic for rapid GBS screening. Swab samples are first eluted and followed by a rapid DNA extraction step where the bacterial cell walls are disrupted by a combination of chemical, physical, and heating effects. The extracted DNA is then subjected to the CRISPR/Cas reaction. The collateral nuclease activity of Cas proteins are activated upon specific binding of gRNA to the atoB gene. Fluorescent signal produced from cleaved probes is captured and indicates the presence of GBS. GBS, group B Streptococcus. gRNA, guide RNA; ssRNA, single-stranded RNA.

Main Article

1These authors contributed equally to this article.

Page created: July 14, 2021
Page updated: August 25, 2021
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