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Volume 22, Number 2—February 2016
Dispatch

Mediterranean Fin Whales (Balaenoptera physalus) Threatened by Dolphin MorbilliVirus

Sandro MazzariolComments to Author , Cinzia Centelleghe, Giorgia Beffagna, Michele Povinelli, Giuliana Terracciano, Cristiano Cocumelli, Antonio Pintore, Daniele Denurra, Cristina Casalone, Alessandra Pautasso, Cristina Esmeralda Di Francesco, and Giovanni Di Guardo
Author affiliations: University of Padova, Padua, Italy (S. Mazzariol, C. Centelleghe, G. Beffagna, M. Povinelli); Istituto Zooprofilattico Sperimentale del Lazio e della Toscana, Rome, Italy (G. Terracciano, C. Cocumelli); Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy (A. Pintore, D. Denurra); Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Turin, Italy (C. Casalone, A. Pautasso); University of Teramo Faculty of Veterinary Medicine, Teramo, Italy (C.E. Di Francesco, G. Di Guardo)

Main Article

Table 2

Primers designed on dolphin morbillivirus isolate used for the total hemagglutinin gene sequence analysis of the virus detected in the newborn fin whale*

Primer Nucleotide position Sense sequence, 5′ → 3′ Fragment length, bp
DMV-10F 7206–7226 GGGTGTGCTAGCCGTTATGT 718
DMV-10R 7904–7924 TTCGTCCTCATCAATCACCA 718
DMV 11F 7799–7891 CCGAACCTGATGATCCATTT 612
DMV-11R 8391–8411 CGTAAATGTCCATCCCTGCT 612
DMV-12F 8290–8309 AACCGGATCCCAGCTTATG 800
DMV-12R 9070–9090 CCAGGTGCACTTCAGGGTAT 800

*Isolate GenBank accession no. AJ608288. Annealing temperature for all primers was 56°C. DMV, dolphin morbillivirus; F, forward; R, reverse.

Main Article

Page created: January 15, 2016
Page updated: January 15, 2016
Page reviewed: January 15, 2016
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