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Volume 24, Number 2—February 2018
Research

Yersinia pestis Survival and Replication in Potential Ameba Reservoir

David W. MarkmanComments to Author , Michael F. Antolin, Richard A. Bowen, William H. Wheat, Michael Woods, Mercedes Gonzalez-Juarrero, and Mary Jackson
Author affiliations: Colorado State University, Fort Collins, Colorado, USA (D.W. Markman, M.F. Antolin, R.A. Bowen, W.H. Wheat, M. Gonzalez-Juarrero, M. Jackson); Burrell College of Osteopathic Medicine, Las Cruces, New Mexico, USA (M. Woods)

Main Article

Table

Properties and kinetics of 5 amebae species after experimental Yersinia pestis infection

Species
 Dormant state
 Infection prevalence*
Infection intensity†
Intracellular survival‡
 Intracellular replication
24 h
48 h
Mean, %
 SEM
 Mean, %
 SEM
 Mean, %
 SEM
 Mean, %
 SEM
Acanthamoeba castellanii Cyst 33.63 5.21 4.22 0.61 0 0 0 0 Inconclusive§
A. lenticulata Cyst 51.66 3.17 6.41 0.43 10 11.55 0 0 No
A. polyphaga Cyst 49.08 5.41 5.36 0.37 31.66 22.04 0 0 No
Dictyostelium discoideum Spore 39.24 3.13 3.57 0.97 270 19.92 226.67 22.71 Yes
Vermamoeba vermiformis Cyst 29.61 3.4 1.84 0.13 10 9.66 0 0 No

*Mean percentage of amebae containing >1 intracellular bacterium.
†Mean no. of intracellular bacteria per individual infected ameba.
‡Mean no. of surviving intracellular bacteria (relative to control) in experiments with 1 and 4 h of antibiotic drug exposure.
§We observed no replication in the intraameba replication assay, which we used to count intra-ameba bacterial colony-forming units before and after co-culture. However, we observed probable but nondefinitive mitotic bacterial replication in the TEM micrographs (Figure 4, panel A).

Main Article

Page created: January 17, 2018
Page updated: January 17, 2018
Page reviewed: January 17, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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