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Volume 29, Number 1—January 2023
Dispatch

Detection of Monkeypox Virus DNA in Airport Wastewater, Rome, Italy

Giuseppina La RosaComments to Author , Pamela Mancini, Carolina Veneri, Giusy Bonanno Ferraro, Luca Lucentini, Marcello Iaconelli, and Elisabetta Suffredini
Author affiliation: Istituto Superiore di Sanità, Rome, Italy

Main Article

Table 1

Primers and probes used to detect monkeypox virus DNA in airport wastewater, Rome, Italy*

PCR ID Target Primer name Primer ID Sequence, 5′ → 3′ Position† Annealing temp. (°C) Amplicon size Ref.
1002 G2R MPVX G F 2368 GGAAAATGTAAAGACAACGAATACAG 194459–84 60 90 bp (12)
MPVX G R 2369 GCTATCACATAATCTGGAAGCGTA 194525–48


MPVX G P
2370
FAM-AAGCCGTAATCTATG TTGTCTATCGTGTCC-BHQ1
194485–514



1005 G2R MPVX G F mod 2377 GGAAAGTGTAAAGACAACGAATACAG 194459–84 60 90 bp (12)
MPVX G R mod 2378 GCTATCACATAATCTGAAAGCGTA 194525–48 This study


MPVX G P
2370
FAM-AAGCCGTAATCTATG TTGTCTATCGTGTCC-BHQ1
194485–514



1003 F3L F3L-F290 2371 CTCATTGATTTTTCGCGGGATA 46313–34 60 107 bp (11)
F3L-R396 2372 GACGATACTCCTCCTCGTTGGT 46398–419


F3Lp333S-MGB
2373
FAM-CATCAGAATCTGTAGGCCGT-MGBNFQ
46398–419



1008 F3L F3L-F290 2371 CTCATTGATTTTTCGCGGGATA 46313–34 60 107 bp (11)
F3L-R396 mod 2384 AACGATACTCCTCCTCGTTGGT 46398–419 This study


F3Lp333S-MGB
2373
FAM-CATCAGAATCTGTAGGCCGT-MGBNFQ
46398–419



1004 N3R N3R-F319 2374 AACAACCGTCCTACAATTAAACAACA 190641–66 60 139 bp (11)
N3R-R457 2375 CGCTATCGAACCATTTTTGTAGTCT 190755–79


N3Rp352S-MGB
2376
FAM-TATAACGGCGAAGAATATACT-MGBNFQ
190674–94



1016 N3R N3R-F319 2374 AACAACCGTCCTACAATTAAACAACA 190641–66 60 139 bp (11)
N3R-R457 2375 CGCTATCGAACCATTTTTGTAGTCT 190755–79 This study


N3Rp352S-MGB mod
2381
FAM-TATAACGGCGACGAATATACT-MGBNFQ
190674–94



1006 G2R G2R-1st cycle F 2379 ATAGCACCACATGCACCATC 194435–54 63 156 bp This study


G2R-1st cycle R
2380
AAAGGTATCCGAACCACACG
194590–71



1005 G2R MPVX G F mod 2377 GGAAAGTGTAAAGACAACGAATACAG 194459–84 61 90 bp This study


MPVX G R mod
2378
GCTATCACATAATCTGAAAGCGTA
194525–48



1009 F3L F3L-1st cycle F 2385 CAGGGTTAACACCTTTCCAA 46242–61 61 212 bp This study


F3L-1st cycle R
2386
TGATCTTCAACGTAGTGCTATGG
46453–31



1008 F3L F3L-F290 2371 CTCATTGATTTTTCGCGGGATA 46313–34 62 107 bp (11)


F3L-R396 mod
2384
AACGATACTCCTCCTCGTTGGT
46398–419


This study
1007 N3R N3R-1st cycle F 2382 TCTATCTCGTTCATGGTCGGTAAT 190503–26 64 455 bp This study


N3R-1st cycle R
2383
CGCACTGTCTTATTCGCCATT
190957–37



1004 N3R N3R-F319 2374 AACAACCGTCCTACAATTAAACAACA 190641–66 64 139 bp (11)
N3R-R457 2375 CGCTATCGAACCATTTTTGTAGTCT 190755–79

*Bold underlined text in bases represents modifications to the original primers and probes. ID, identification; F, forward; mod, modified; MPXV, monkeypox virus; R, reverse; ref., reference. †Position based on monkeypox virus reference isolate MPXV_USA_2022_MA001, complete genome, GenBank accession no. ON563414.

Main Article

Page created: November 15, 2022
Page updated: December 22, 2022
Page reviewed: December 22, 2022
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