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Volume 25, Number 5—May 2019

Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues

Jennifer Chua1Comments to Author , Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. CoteComments to Author 
Author affiliations: United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA

Main Article

Table 6

Effect of residual electron microscopy fixative in tissue from guinea pigs on the growth of Bacillus anthracis Sterne in study of inactivation of bacterial Tier I Select Agents*

Tissue type, no. samples Fixation time, d Incubation time for tissue in PBS/water, h Sample inactivation† Positive control
Spleen, 3 14 24 –/– +/+
Spleen, 3 21 24 –/– +/+
Liver, 3 14 24 –/– +/+
Liver, 3 21 24 –/– +/+

*Spleen and liver tissues fixed for 14 or 21 d and washed for 24 h permitted Sterne spores to germinate and grow in broth used for viability testing. +/− indicates presence and/or absence of growth in broth/on plates. PBS, phosphate-buffered saline.
†50% of tissue tested.

Main Article

1These authors contributed equally to this article.

Page created: April 18, 2019
Page updated: April 18, 2019
Page reviewed: April 18, 2019
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