Volume 25, Number 5—May 2019
Research
Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues
Jennifer Chua1
, Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. Cote
, Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. Cote
Table 6
Effect of residual electron microscopy fixative in tissue from guinea pigs on the growth of Bacillus anthracis Sterne in study of inactivation of bacterial Tier I Select Agents*
| Tissue type, no. samples | Fixation time, d | Incubation time for tissue in PBS/water, h | Sample inactivation† | Positive control |
|---|---|---|---|---|
| Spleen, 3 | 14 | 24 | –/– | +/+ |
| Spleen, 3 | 21 | 24 | –/– | +/+ |
| Liver, 3 | 14 | 24 | –/– | +/+ |
| Liver, 3 | 21 | 24 | –/– | +/+ |
*Spleen and liver tissues fixed for 14 or 21 d and washed for 24 h permitted Sterne spores to germinate and grow in broth used for viability testing. +/− indicates presence and/or absence of growth in broth/on plates. PBS, phosphate-buffered saline.
†50% of tissue tested.
1These authors contributed equally to this article.
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