Volume 25, Number 5—May 2019
Research
Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues
Jennifer Chua1
, Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. Cote
, Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. Cote
Table 7
Growth of Bacillus, Burkholderia, Francisella, and Yersinia species in diluted formaldehyde in study of inactivation of bacterial Tier I Select Agents*
| Strain | CFU/volume | Growth condition | Time, d | NBF concentration, % | Growth |
|---|---|---|---|---|---|
| Bacillus anthracis Sterne |
5 (bacilli)/500 mL |
BHI, 37°C |
1 |
0.1 | – |
| 0.01 | + | ||||
| 0.001 |
+ |
||||
| Burkholderia pseudomallei 82 |
10/500 mL |
LB + 4% glycerol, 37°C |
5 |
0.1 | – |
| 0.01 | – | ||||
| 0.001 |
+ |
||||
| B. pseudomallei 1026b |
5/500 mL |
LB + 4% glycerol, 37°C |
3 |
0.1 | – |
| 0.01 | + | ||||
| 0.001 |
+ |
||||
| Francisella tularensis LVS |
1.4 × 105/100 mL |
BHI + 1% IsoVitaleX, 37°C |
4 |
0.1 | – |
| 0.01 | – | ||||
| 0.001 |
+ |
||||
| Yersina pestis Pgm–/pPst– | 10/100 mL | HI + 0.2% xylose, 28°C | 3 | 0.1 | – |
| 0.01 | – | ||||
| 0.001 | + |
*5 CFU B. anthracis Sterne (bacilli), 10 CFU B. pseudomallei 82, 5 CFU B. pseudomallei 1026b, 1.4 × 105 CFU F. tularensis live vaccine strain and 10 CFU Y. pestis Pgm–/pPst– bacteria were inoculated into their respective broths containing 0.1%, 0.01%, and 0.001% NBF and grown at the indicated temperature. Time listed is when broth cultures reached turbidity. + /– indicates presence and/or absence of growth in broth.
BHI, brain heart infusion; HI, heart infusion; LB, Lennox broth; LVS, live vaccine strain; NBF, neutral buffered formalin.
1These authors contributed equally to this article.
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Page updated: April 18, 2019
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